PLoS biology, 2021-04, Vol.19 (4), p.e3001194-e3001194
2021
Details
- Autor(en) / Beteiligte
- Titel
- Bacterial persisters are a stochastically formed subpopulation of low-energy cells
- Ist Teil von
- PLoS biology, 2021-04, Vol.19 (4), p.e3001194-e3001194
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Persisters represent a small subpopulation of non- or slow-growing bacterial cells that are tolerant to killing by antibiotics. Despite their prominent role in the recalcitrance of chronic infections to antibiotic therapy, the mechanism of their formation has remained elusive. We show that sorted cells of Escherichia coli with low levels of energy-generating enzymes are better able to survive antibiotic killing. Using microfluidics time-lapse microscopy and a fluorescent reporter for in vivo ATP measurements, we find that a subpopulation of cells with a low level of ATP survives killing by ampicillin. We propose that these low ATP cells are formed stochastically as a result of fluctuations in the abundance of energy-generating components. These findings point to a general "low energy" mechanism of persister formation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1545-7885, 1544-9173eISSN: 1545-7885DOI: 10.1371/journal.pbio.3001194Titel-ID: cdi_plos_journals_2528202346
- Format
- –
- Schlagworte
- Abbreviations, Anti-Bacterial Agents - pharmacology, Antibiotics, Antitoxins, ATP, Bacteria - drug effects, Bacteria - genetics, Bacteria - growth & development, Bacteria - metabolism, Bacterial Physiological Phenomena - drug effects, Biology and Life Sciences, Carbon, Citric Acid Cycle - drug effects, Colonies, Computer and Information Sciences, Dehydrogenases, Drug Resistance, Multiple, Bacterial - drug effects, E coli, Energy, Energy Metabolism - drug effects, Energy Metabolism - physiology, Engineering and Technology, Enzymes, Escherichia coli - drug effects, Escherichia coli - growth & development, Escherichia coli - metabolism, Flow cytometry, Fluorescence, Isocitrate dehydrogenase, Kinases, Krebs cycle, Medicine and Health Sciences, Microbial Sensitivity Tests, Microbial Viability - drug effects, Organisms, Genetically Modified, Physical Sciences, Population, Protein transport, Research and Analysis Methods, Short Reports, Stationary phase, Toxins, Tricarboxylic acid cycle, tRNA, Urinary tract, Yellow fluorescent protein