PLoS biology, 2020-09, Vol.18 (9), p.e3000825-e3000825
2020
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- Autor(en) / Beteiligte
- Titel
- Porphyromonas gingivalis promotes progression of esophageal squamous cell cancer via TGFβ-dependent Smad/YAP/TAZ signaling
- Ist Teil von
- PLoS biology, 2020-09, Vol.18 (9), p.e3000825-e3000825
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Microbial dysbiosis in the upper digestive tract is linked to an increased risk of esophageal squamous cell carcinoma (ESCC). Overabundance of Porphyromonas gingivalis is associated with shorter survival of ESCC patients. We investigated the molecular mechanisms driving aggressive progression of ESCC by P. gingivalis. Intracellular invasion of P. gingivalis potentiated proliferation, migration, invasion, and metastasis abilities of ESCC cells via transforming growth factor-β (TGFβ)-dependent Drosophila mothers against decapentaplegic homologs (Smads)/Yes-associated protein (YAP)/Transcriptional coactivator with PDZ-binding motif (TAZ) activation. Smads/YAP/TAZ/TEA domain transcription factor1 (TEAD1) complex formation was essential to initiate downstream target gene expression, inducing an epithelial-mesenchymal transition (EMT) and stemness features. Furthermore, P. gingivalis augmented secretion and bioactivity of TGFβ through glycoprotein A repetitions predominant (GARP) up-regulation. Accordingly, disruption of either the GARP/TGFβ axis or its activated Smads/YAP/TAZ complex abrogated the tumor-promoting role of P. gingivalis. P. gingivalis signature genes based on its activated effector molecules can efficiently distinguish ESCC patients into low- and high-risk groups. Targeting P. gingivalis or its activated effectors may provide novel insights into clinical management of ESCC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1545-7885, 1544-9173eISSN: 1545-7885DOI: 10.1371/journal.pbio.3000825Titel-ID: cdi_plos_journals_2451552196
- Format
- –
- Schlagworte
- Acyltransferases, Adaptor Proteins, Signal Transducing - metabolism, Adult, Aged, Animals, Bacteroidaceae Infections - complications, Bacteroidaceae Infections - metabolism, Bacteroidaceae Infections - mortality, Bacteroidaceae Infections - pathology, Biological activity, Biology and life sciences, Cancer, Cell proliferation, Cells, Cultured, Clinical outcomes, Colorectal cancer, Complex formation, Dentistry, Digestive tract, Disease Progression, Drosophila, Dysbacteriosis, Epigenetics, Esophageal cancer, Esophageal Neoplasms - metabolism, Esophageal Neoplasms - microbiology, Esophageal Neoplasms - mortality, Esophageal Neoplasms - pathology, Esophageal Squamous Cell Carcinoma - metabolism, Esophageal Squamous Cell Carcinoma - microbiology, Esophageal Squamous Cell Carcinoma - mortality, Esophageal Squamous Cell Carcinoma - pathology, Esophagus, Female, Follow-Up Studies, Funding, Gene expression, Glycoproteins, Growth factors, HCT116 Cells, Homology, Hospitals, Humans, Immunology, Infections, Infectious diseases, Laboratories, Male, Medical prognosis, Medical schools, Medicine, Medicine and Health Sciences, Mesenchyme, Metastases, Metastasis, Mice, Mice, Inbred BALB C, Mice, Nude, Microbiota, Microorganisms, Middle Aged, Molecular modelling, Oral cancer, Porphyromonas gingivalis, Porphyromonas gingivalis - physiology, Risk groups, Signal Transduction - physiology, Smad protein, Smad Proteins - metabolism, Squamous cell carcinoma, Survival Analysis, Thoracic surgery, Transcription Factors - metabolism, Transforming Growth Factor beta - metabolism, Transforming Growth Factor beta - physiology, Transforming growth factor-b, YAP-Signaling Proteins, Yes-associated protein