Details

Autor(en) / Beteiligte

• Budnick, James A
• Sheehan, Lauren M
• Benton, Angela H
• Pitzer, Joshua E
• Kang, Lin
• Michalak, Pawel
• Roop, R. Martin
• Caswell, Clayton C
• Cloeckaert, Axel

Titel

Characterizing the transport and utilization of the neurotransmitter GABA in the bacterial pathogen Brucella abortus

Ist Teil von

• PloS one, 2020-08, Vol.15 (8), p.e0237371-e0237371

Ort / Verlag

San Francisco: Public Library of Science

Erscheinungsjahr

2020

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The neurotransmitter gamma-aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the human brain; however, it is becoming more evident that this non-proteinogenic amino acid plays multiple physiological roles in biology. In the present study, the transport and function of GABA is studied in the highly infectious intracellular bacterium Brucella abortus. The data show that .sup.3 H-GABA is imported by B. abortus under nutrient limiting conditions and that the small RNAs AbcR1 and AbcR2 negatively regulate this transport. A specific transport system, gts, is responsible for the transport of GABA as determined by measuring .sup.3 H-GABA transport in isogenic deletion strains of known AbcR1/2 regulatory targets; however, this locus is unnecessary for Brucella infection in BALB/c mice. Similar assays revealed that .sup.3 H-GABA transport is uninhibited by the 20 standard proteinogenic amino acids, representing preference for the transport of .sup.3 H-GABA. Metabolic studies did not show any potential metabolic utilization of GABA by B. abortus as a carbon or nitrogen source, and RNA sequencing analysis revealed limited transcriptional differences between B. abortus 2308 with or without exposure to GABA. While this study provides evidence for GABA transport by B. abortus, questions remain as to why and when this transport is utilized during Brucella pathogenesis.