PloS one, 2020-08, Vol.15 (8), p.e0237569-e0237569
2020
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Details
- Autor(en) / Beteiligte
- Titel
- A novel chloroplast super-complex consisting of the ATP synthase and photosystem I reaction center
- Ist Teil von
- PloS one, 2020-08, Vol.15 (8), p.e0237569-e0237569
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2020
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Several 'super-complexes' of individual hetero-oligomeric membrane protein complexes, whose function is to facilitate intra-membrane electron and proton transfer and harvesting of light energy, have been previously characterized in the mitochondrial cristae and chloroplast thylakoid membranes. We report the presence of an intra-membrane super-complex dominated by the ATP-synthase, photosystem I (PSI) reaction-center complex and the ferredoxin-NADP+ Reductase (FNR) in the thylakoid membrane. The presence of the super-complex has been documented by mass spectrometry, clear-native PAGE and Western Blot analyses. This is the first documented presence of ATP synthase in a super-complex with the PSI reaction-center located in the non-appressed stromal domain of the thylakoid membrane.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0237569Titel-ID: cdi_plos_journals_2435753662
- Format
- –
- Schlagworte
- Adenosine Triphosphate - metabolism, ATP, ATP synthase, ATPases, BASIC BIOLOGICAL SCIENCES, Biology and Life Sciences, Chlorophyll, Chloroplasts, Chloroplasts - metabolism, Composition, Cristae, Cytochrome, Discovery and exploration, Electron Transport, Energy, Energy harvesting, Ferredoxin, Ferredoxin-NADP Reductase - metabolism, Geffen, David, Laboratories, Mass spectrometry, Mass spectroscopy, Membrane proteins, Membranes, Mitochondria, NADP, Neurosciences, Nitric Oxide Synthase - metabolism, Photosynthesis, Photosystem I, Photosystem I Protein Complex - metabolism, Photosystem II Protein Complex - metabolism, Physical Sciences, Plant Leaves - metabolism, Plant Proteins - metabolism, Proteins, Proton-Translocating ATPases - metabolism, Reductases, Research and analysis methods, Science & Technology - Other Topics, Scientific imaging, Semel, Terry, Spinacia oleracea - growth & development, Spinacia oleracea - metabolism, Sucrose, Thylakoid membranes, Thylakoids - metabolism