PloS one, 2020-05, Vol.15 (5), p.e0233394
2020
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Details
- Autor(en) / Beteiligte
- Titel
- Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development
- Ist Teil von
- PloS one, 2020-05, Vol.15 (5), p.e0233394
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2020
- Quelle
- Elektronische Zeitschriftenbibliothek (Open access)
- Beschreibungen/Notizen
- Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Here we show that contrary to Chd1-8 disruption, Chd9-/-animals are viable, fertile and display no developmental defects or disease predisposition. Germline deletion of Chd9 only moderately affects gene expression in tissues and derived cells, whereas acute depletion in human cancer cells elicits more robust changes suggesting that CHD9 is a highly context-dependent chromatin regulator that, surprisingly, is dispensable for mouse development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0233394Titel-ID: cdi_plos_journals_2406921481
- Format
- –
- Schlagworte
- Analysis, Animals, ATPases, B cells, Binding proteins, Biochemistry, Biology and Life Sciences, Cancer, Cancer cells, Chromatin, Coronary heart disease, Deoxyribonucleic acid, Depletion, Deregulation, Diseases, Displays (Marketing), Disruption, DNA, DNA binding proteins, DNA helicase, DNA repair, DNA-binding protein, Embryogenesis, Embryonic development, Embryonic growth stage, Enzymes, Fate, Fluorides, Gene deletion, Gene expression, Gene regulation, Genes, Genetic aspects, Genetic regulation, Health aspects, Medical prognosis, Medicine and Health Sciences, Mutagenesis, Mutation, Phenotypes, Protein binding, Proteins, Research and Analysis Methods, RNA polymerase, Time, Transcription, Transcription (Genetics), Transcription factors