PLoS biology, 2020-01, Vol.18 (1), p.e3000567-e3000567
2020
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Details
- Autor(en) / Beteiligte
- Titel
- Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila
- Ist Teil von
- PLoS biology, 2020-01, Vol.18 (1), p.e3000567-e3000567
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2020
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount's capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1545-7885, 1544-9173eISSN: 1545-7885DOI: 10.1371/journal.pbio.3000567Titel-ID: cdi_plos_journals_2355996851
- Format
- –
- Schlagworte
- Abdomen, Age Factors, Anatomy, Cross-Sectional - methods, Animals, Bioengineering, Biology and Life Sciences, Carbon dioxide, Colonization, Correlation analysis, Digestive system, Digestive tract, Drosophila, Drosophila - anatomy & histology, Drosophila - microbiology, Earth Sciences, Ecology and Environmental Sciences, Gastrointestinal Microbiome, Imaging, Insects, Intestinal microflora, Intestine, Intestines - anatomy & histology, Intestines - diagnostic imaging, Intravital Microscopy - methods, Longitudinal studies, Medicine, Medicine and Health Sciences, Methods and Resources, Microbiota, Microorganisms, Microscopy, Optical Imaging - methods, Organs, Physical Sciences, Physiology, Proteins, Research and Analysis Methods, Stem cells, Universal Serial Bus, Viscera - anatomy & histology, Viscera - diagnostic imaging