PloS one, 2020-02, Vol.15 (2), p.e0227586-e0227586
2020
Details
- Autor(en) / Beteiligte
- Titel
- Expression of the muscle-associated gene MYF6 in hairy cell leukemia
- Ist Teil von
- PloS one, 2020-02, Vol.15 (2), p.e0227586-e0227586
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Hairy cell leukemia (HCL) is a purine analog-responsive B-cell malignancy containing the BRAF V600E mutation, expressing CD22, CD11c, CD103, tartrate resistant acid phosphatase (TRAP) CD25, CD123, and annexin 1A. BRAF V600E and the latter 4 markers are usually absent in the more aggressive and chemoresistant variant HCLv. To evaluate differences between HCL and HCLv, expression microarrays comparing HCL with HCLv were performed for 24694 genes using 47323 probes. Microarray data from 35 HCL and 27 HCLv purified samples showed the greatest HCL-HCLv difference in the muscle-associated gene MYF6, expressed by its 2 probes 18.5- and 10.8-fold higher in HCL than HCLv (p<0.0001). By real-time quantitative PCR (RQ-PCR), 100% of 152 classic HCL samples were MYF6-positive, vs 5 (6%) of 90 blood donors. MYF6-expression was also detected in 18 (35%) of 51 with HCLv, 11 (92%) of 12 with HCL expressing unmutated IGHV4-34, 35 (73%) of 48 with chronic lymphocytic leukemia (CLL), and 1 (8%) of 12 with mantle cell lymphoma. Hypomethylation status of MYF6 supported expression in HCL more than HCLv. Posttreatment blood samples becoming negative by flow cytometry remained MYF6+ by RQ-PCR in 42 (48%) of 87 HCL patients, and MYF6 RQ-PCR could detect 1 HCL in 105 normal cells. MYF6, universally expressed in HCL and in most CLL samples, may be a useful biomarker for these leukemias. Further studies are underway to determine the role of MYF6 in HCL.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0227586Titel-ID: cdi_plos_journals_2352995024
- Format
- –
- Schlagworte
- Acid phosphatase, Acid phosphatase (tartrate-resistant), Acid resistance, Adult, Aged, Alkaloids, B cells, Biology and Life Sciences, Biomarkers, Biotechnology industries, Biotechnology industry, Blood, Blood donation, Blood donors, Cancer, Cancer genetics, CD103 antigen, CD11c antigen, CD123 antigen, CD22 antigen, CD25 antigen, Chronic lymphatic leukemia, Chronic lymphocytic leukemia, Consent, DNA Methylation - genetics, DNA microarrays, EDTA, Ethylenediaminetetraacetic acid, Female, Flow cytometry, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Leukemic, Gene mutation, Gene mutations, Genes, Genetic aspects, Hairy cell leukemia, Humans, Laboratories, Leukemia, Leukemia, Hairy cell, Leukemia, Hairy Cell - genetics, Leukemia, Lymphocytic, Chronic, B-Cell - genetics, Limit of Detection, Lymphatic leukemia, Lymphocytes B, Lymphocytic leukemia, Lymphoma, Lymphomas, Male, Malignancy, Mantle cell lymphoma, Medical prognosis, Medicine and Health Sciences, Middle Aged, Molecular biology, Muscles, Muscles - metabolism, Musculoskeletal system, Mutation, Myogenic Regulatory Factors - genetics, Myogenic Regulatory Factors - metabolism, Neoplasm, Residual - genetics, Neoplasm, Residual - pathology, Pathology, Phosphatases, Polymerase chain reaction, Probes, Research and Analysis Methods, Time, Tumors