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An increasing number of citizen science water monitoring programs is continuously collecting water quality data on streams throughout the United States. Operating under quality assurance protocols, this type of monitoring data can be extremely valuable for scientists and professional agencies, but in some cases has been of limited use due to concerns about the accuracy of data collected by volunteers. Although a growing body of studies attempts to address accuracy concerns by comparing volunteer data to professional data, rarely has this been conducted with large-scale datasets generated by citizen scientists. This study assesses the relative accuracy of volunteer water quality data collected by the Texas Stream Team (TST) citizen science program from 1992-2016 across the State of Texas by comparing it to professional data from corresponding stations during the same time period. Use of existing data meant that sampling times and protocols were not controlled for, thus professional and volunteer comparisons were refined to samples collected at stations within 60 meters of one another and during the same year. Results from the statewide TST dataset include 82 separate station/year ANOVAs and demonstrate that large-scale, existing volunteer and professional data with unpaired samples can show agreement of ~80% for all analyzed parameters (DO = 77%, pH = 79%, conductivity = 85%). In addition, to assess whether limiting variation within the source datasets increased the level of agreement between volunteers and professionals, data were analyzed at a local scale. Data from a single partner city, with increased controls on sampling times and locations and correction of a systematic bias in DO, confirmed this by showing an even greater agreement of 91% overall from 2009-2017 (DO = 91%, pH = 83%, conductivity = 100%). An experimental sampling dataset was analyzed and yielded similar results, indicating that existing datasets can be as accurate as experimental datasets designed with researcher supervision. Our findings underscore the reliability of large-scale citizen science monitoring datasets already in existence, and their potential value to scientific research and water management programs.