PLoS pathogens, 2019-11, Vol.15 (11), p.e1008146-e1008146
2019
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- Autor(en) / Beteiligte
- Titel
- 14-3-3 scaffold proteins mediate the inactivation of trim25 and inhibition of the type I interferon response by herpesvirus deconjugases
- Ist Teil von
- PLoS pathogens, 2019-11, Vol.15 (11), p.e1008146-e1008146
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The 14-3-3 molecular scaffolds promote type I interferon (IFN) responses by stabilizing the interaction of RIG-I with the TRIM25 ligase. Viruses have evolved unique strategies to halt this cellular response to support their replication and spread. Here, we report that the ubiquitin deconjugase (DUB) encoded in the N-terminus of the Epstein-Barr virus (EBV) large tegument protein BPLF1 harnesses 14-3-3 molecules to promote TRIM25 autoubiquitination and sequestration of the ligase into inactive protein aggregates. Catalytically inactive BPLF1 induced K48-linked autoubiquitination and degradation of TRIM25 while the ligase was mono- or di-ubiquitinated in the presence of the active viral enzyme and formed cytosolic aggregates decorated by the autophagy receptor p62/SQSTM1. Aggregate formation and the inhibition of IFN response were abolished by mutations of solvent exposed residues in helix-2 of BPLF1 that prevented binding to 14-3-3 while preserving both catalytic activity and binding to TRIM25. 14-3-3 interacted with the Coiled-Coil (CC) domain of TRIM25 in in vitro pulldown, while BPLF1 interacted with both the CC and B-box domains, suggesting that 14-3-3 positions BPLF1 at the ends of the CC dimer, close to known autoubiquitination sites. Our findings provide a molecular understanding of the mechanism by which a viral deubiquitinase inhibits the IFN response and emphasize the role of 14-3-3 proteins in modulating antiviral defenses.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7374, 1553-7366eISSN: 1553-7374DOI: 10.1371/journal.ppat.1008146Titel-ID: cdi_plos_journals_2327518641
- Format
- –
- Schlagworte
- 14-3-3 protein, 14-3-3 Proteins - genetics, 14-3-3 Proteins - metabolism, Aggregates, Antiviral Agents - pharmacology, Autophagy, Binding, Binding sites, Biological response modifiers, Biology and Life Sciences, Biotechnology industries, Catalytic activity, Chromatin, Coils, Deactivation, Dimers, Domains, Enzymes, Epstein-Barr virus, Harnesses, HeLa Cells, Herpesviridae - drug effects, Herpesviridae - immunology, Herpesviridae Infections - drug therapy, Herpesviridae Infections - immunology, Herpesviridae Infections - metabolism, Herpesviridae Infections - virology, Humans, Immunity, Innate - drug effects, Immunity, Innate - immunology, Inactivation, Infectious diseases, Interferon, Interferon Type I - pharmacology, Kinases, Laboratories, Ligases, Medicin och hälsovetenskap, Molecular biology, Mutation, N-Terminus, Phagocytosis, Physical Sciences, Plasmids, Polypeptides, Protein Binding, Proteins, Proteolysis, Research and analysis methods, Scaffolds, Signal Transduction, Tegument, Transcription Factors - genetics, Transcription Factors - metabolism, Tripartite Motif Proteins - genetics, Tripartite Motif Proteins - metabolism, Ubiquitin, Ubiquitin - metabolism, Ubiquitin-Protein Ligases - genetics, Ubiquitin-Protein Ligases - metabolism, Ubiquitination, Viral infections, Viral Regulatory and Accessory Proteins - genetics, Viral Regulatory and Accessory Proteins - metabolism, Virus Replication, Viruses