Details

Autor(en) / Beteiligte

• Cêtre-Sossah, Catherine
• Pédarrieu, Aurélie
• Juremalm, Mikael
• Jansen Van Vuren, Petrus
• Brun, Alejandro
• Ould El Mamy, Ahmed Bezeid
• Héraud, Jean-Michel
• Filippone, Claudia
• Ravalohery, Jean-Pierre
• Chaabihi, Hassan
• Albina, Emmanuel
• Dommergues, Laure
• Paweska, Janusz
• Cardinale, Eric
• Al-Salem, Waleed Saleh

Titel

Development and validation of a pen side test for Rift Valley fever

Ist Teil von

• PLoS neglected tropical diseases, 2019-09, Vol.13 (9), p.e0007700-e0007700

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Rift Valley fever (RVF) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures. A first-line lateral flow immunochromatographic strip test (LFT) was developed for the detection of the nucleoprotein (N) of the RVF virus (RVFV). Its diagnostic performance characteristics were evaluated using reference stocks isolates recovered from different hosts and in geographic regions mimicking clinical specimens and from known RVF negative serum samples. A high level of diagnostic accuracy (DSe (35/35), DSp (167/169)) was observed, including the absence of cross-reactivity with viruses belonging to different genera. The fact no specialized reagents and laboratory equipment are needed, make this assay a valuable, first-line diagnostic tool in resource-poor diagnostic territories for on-site RVFV detection, however the staff require training.