The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
2019
Details
- Autor(en) / Beteiligte
- Titel
- The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
- Ist Teil von
- PLoS genetics, 2019-08, Vol.15 (8), p.e1008316-e1008316
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek (Open access)
- Beschreibungen/Notizen
- The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a 'zipper'-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acetylated histones for degradation during somatic DNA double strand break repair and during histone replacement during spermiogenesis. We investigated the role of the testis-specific proteasomal subunit α4s (PSMA8) during spermatogenesis, and found that PSMA8 was localized to and dependent on the central region of the synaptonemal complex. Accordingly, synapsis-deficient mice show delocalization of PSMA8. Moreover, though Psma8-deficient mice are proficient in meiotic homologous recombination, there are alterations in the proteostasis of several key meiotic players that, in addition to the known substrate acetylated histones, have been shown by a proteomic approach to interact with PSMA8, such as SYCP3, SYCP1, CDK1 and TRIP13. These alterations lead to an accumulation of spermatocytes in metaphase I and II which either enter massively into apoptosis or give rise to a low number of aberrant round spermatids that apoptose before histone replacement takes place.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7404, 1553-7390eISSN: 1553-7404DOI: 10.1371/journal.pgen.1008316Titel-ID: cdi_plos_journals_2291478884
- Format
- –
- Schlagworte
- Analysis, Animal spermatogenesis, Animals, Apoptosis, Apoptosis - genetics, Biology, Biology and Life Sciences, Cell cycle, Chromosomes, Deoxyribonucleic acid, Disease Models, Animal, DNA, DNA damage, DNA repair, Female, Fertility, Fertility - genetics, Genetic aspects, Genetics, Genomes, Histones, Homologous recombination, Infertility, Male - genetics, Influence, Male, Meiosis, Metaphase, Metaphase - genetics, Mice, Mice, Knockout, Nuclear Proteins - metabolism, Prophase, Proteasome activator, Proteasome Endopeptidase Complex - genetics, Proteasome Endopeptidase Complex - metabolism, Proteasomes, Protein Subunits - genetics, Protein Subunits - metabolism, Proteins, Research and Analysis Methods, Spermatids, Spermatocytes, Spermatocytes - metabolism, Spermatogenesis, Spermatogenesis - genetics, Spermatogenesis in animals, Spermiogenesis, Synaptonemal complex, Synaptonemal Complex - metabolism, Testis, Testis - cytology, Testis - metabolism, Ubiquitin, Ubiquitin-proteasome system