PloS one, 2019-08, Vol.14 (8), p.e0221975-e0221975
2019
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- Autor(en) / Beteiligte
- Titel
- Crystal structure of L-aspartate aminotransferase from Schizosaccharomyces pombe
- Ist Teil von
- PloS one, 2019-08, Vol.14 (8), p.e0221975-e0221975
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- L-aspartate aminotransferase is a pyridoxal 5'-phosphate-dependent transaminase that catalyzes reversible transfer of an α-amino group from aspartate to α-ketoglutarate or from glutamate to oxaloacetate. L-aspartate aminotransferase not only mediates amino acid and carbohydrate metabolism but also regulates the cellular level of amino acids by catalyzing amino acid degradation and biosynthesis. To expand our structural information, we determined the crystal structure of L-aspartate aminotransferase from Schizosaccharomyces pombe at 2.1 Å resolution. A structural comparison between two yeast L-aspartate aminotransferases revealed conserved enzymatic mechanism mediated by the open-closed conformational change. Compared with higher eukaryotic species, L-aspartate aminotransferases showed distinguishable inter-subunit interaction between the N-terminal arm and a large domain of the opposite subunit. Interestingly, structural homology search showed varied conformation of the N-terminal arm among 71 structures of the family. Therefore, we classified pyridoxal 5'-phosphate-dependent enzymes into eight subclasses based on the structural feature of N-terminal arms. In addition, structure and sequence comparisons showed strong relationships among the eight subclasses. Our results may provide insights into structure-based evolutionary aspects of pyridoxal 5'-phosphate-dependent enzymes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0221975Titel-ID: cdi_plos_journals_2282685132
- Format
- –
- Schlagworte
- Amino acids, Ascomycota, Aspartate, Aspartate aminotransferase, Aspartate Aminotransferases - chemistry, Binding Sites, Biology and Life Sciences, Biosynthesis, Carbohydrate metabolism, Carbohydrates, Catalytic Domain, Computer and Information Sciences, Conformation, Crystal structure, Crystallization, E coli, Enzymes, Evolution, Molecular, Family, Glutamate, Homology, Ketoglutaric acid, Metabolism, Models, Molecular, Mutation, Phosphates, Physical Sciences, Protein Binding, Protein Conformation, Protein Interaction Domains and Motifs, Proteins, Research and Analysis Methods, Schizosaccharomyces pombe, Schizosaccharomyces pombe Proteins - chemistry, Structure-Activity Relationship, Transaminase, Transaminases, Yeast, Yeasts