PLoS pathogens, 2019-01, Vol.15 (1), p.e1007538-e1007538
2019
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- Autor(en) / Beteiligte
- Titel
- Urease is an essential component of the acid response network of Staphylococcus aureus and is required for a persistent murine kidney infection
- Ist Teil von
- PLoS pathogens, 2019-01, Vol.15 (1), p.e1007538-e1007538
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Staphylococcus aureus causes acute and chronic infections resulting in significant morbidity. Urease, an enzyme that generates NH3 and CO2 from urea, is key to pH homeostasis in bacterial pathogens under acidic stress and nitrogen limitation. However, the function of urease in S. aureus niche colonization and nitrogen metabolism has not been extensively studied. We discovered that urease is essential for pH homeostasis and viability in urea-rich environments under weak acid stress. The regulation of urease transcription by CcpA, Agr, and CodY was identified in this study, implying a complex network that controls urease expression in response to changes in metabolic flux. In addition, it was determined that the endogenous urea derived from arginine is not a significant contributor to the intracellular nitrogen pool in non-acidic conditions. Furthermore, we found that during a murine chronic renal infection, urease facilitates S. aureus persistence by promoting bacterial fitness in the low-pH, urea-rich kidney. Overall, our study establishes that urease in S. aureus is not only a primary component of the acid response network but also an important factor required for persistent murine renal infections.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7374, 1553-7366eISSN: 1553-7374DOI: 10.1371/journal.ppat.1007538Titel-ID: cdi_plos_journals_2251133712
- Format
- –
- Schlagworte
- Acids, Acids - metabolism, Amino acids, Ammonia, Animals, Arginine, Bacterial Proteins - metabolism, Biofilms, Biology and Life Sciences, Carbon dioxide, Care and treatment, Cell death, Chronic infection, Colonization, Enzymes, Female, Fitness, Funding, Gene expression, Gene regulation, Homeostasis, Homeostasis - physiology, Hydrogen-Ion Concentration, Hydrolases, Infection, Kidney - microbiology, Kidney diseases, Kidney Diseases - microbiology, Kidneys, Male, Medicine and Health Sciences, Metabolic flux, Metabolism, Mice, Mice, Inbred C57BL, Morbidity, Nitrogen, Nitrogen - metabolism, Pathogenic microorganisms, Pathogens, Pathology, Persistent infection, pH effects, Physical Sciences, Physiology, Risk factors, Signal transduction, Staphylococcal infections, Staphylococcal Infections - metabolism, Staphylococcus aureus, Staphylococcus aureus - metabolism, Staphylococcus aureus - pathogenicity, Staphylococcus aureus infections, Staphylococcus infections, Supervision, Transcription, Urea, Urea - metabolism, Ureas, Urease, Urease - genetics, Urease - metabolism, Urease - physiology, Viability