PLoS genetics, 2019-05, Vol.15 (5), p.e1008176
2019
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- Autor(en) / Beteiligte
- Titel
- CREB-B acts as a key mediator of NPF/NO pathway involved in phase-related locomotor plasticity in locusts
- Ist Teil von
- PLoS genetics, 2019-05, Vol.15 (5), p.e1008176
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Gene expression changes in neural systems are essential for environment-induced behavioral plasticity in animals; however, neuronal signaling pathways mediating the effect of external stimuli on transcriptional changes are largely unknown. Recently, we have demonstrated that the neuropeptide F (NPF)/nitric oxide (NO) signaling pathway plays a regulatory role in phase-related locomotor plasticity in the migratory locust, Locusta migratoria. Here, we report that a conserved transcription factor, cAMP response element-binding protein B (CREB-B), is a key mediator involved in the signaling pathway from NPF2 to NOS in the migratory locust, triggering locomotor activity shift between solitarious and gregarious phases. We find that CREB-B directly activates brain NOS expression by interacting with NOS promoter region. The phosphorylation at serine 110 site of CREB-B dynamically changes in response to population density variation and is negatively controlled by NPF2. The involvement of CREB-B in NPF2-regulated locomotor plasticity is further validated by RNAi experiment and behavioral assay. Furthermore, we reveal that protein kinase A mediates the regulatory effects of NPF2 on CREB-B phosphorylation and NOS transcription. These findings highlight a precise signal cascade underlying environment-induced behavioral plasticity.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7404, 1553-7390eISSN: 1553-7404DOI: 10.1371/journal.pgen.1008176Titel-ID: cdi_plos_journals_2251079319
- Format
- –
- Schlagworte
- Animal behavior, Animal locomotion, Animals, Behavioral plasticity, Biochemistry, Biology and Life Sciences, Cellular signal transduction, Cyclic adenosine monophosphate, Cyclic AMP response element-binding protein, Cyclic AMP Response Element-Binding Protein - genetics, Cyclic AMP Response Element-Binding Protein - metabolism, Cyclic AMP-Dependent Protein Kinases - genetics, Cyclic AMP-Dependent Protein Kinases - metabolism, External stimuli, Gene expression, Gene Expression Regulation - genetics, Genetic aspects, Grasshoppers - genetics, Grasshoppers - metabolism, Insects, Kinases, Locomotion - genetics, Locomotor activity, Locusta migratoria - genetics, Locusta migratoria - metabolism, Locusts (Insects), Medicine and Health Sciences, Neuronal Plasticity - genetics, Neurons, Neuropeptide F, Neuropeptides, Neuropeptides - metabolism, Neuropeptides - physiology, Nitric oxide, Nitric Oxide - metabolism, Nitric Oxide - physiology, Nitric-oxide synthase, Nitrogen oxides, Phosphorylation, Physiological aspects, Plasticity (neural), Population density, Promoter Regions, Genetic - genetics, Protein B, Protein binding, Protein kinase A, Protein kinases, RNA Interference, RNA-mediated interference, Serine, Serine - metabolism, Signal Transduction