Details

Autor(en) / Beteiligte

• Pokusaeva, Victoria O
• Usmanova, Dinara R
• Putintseva, Ekaterina V
• Espinar, Lorena
• Sarkisyan, Karen S
• Mishin, Alexander S
• Bogatyreva, Natalya S
• Ivankov, Dmitry N
• Akopyan, Arseniy V
• Avvakumov, Sergey Ya
• Povolotskaya, Inna S
• Filion, Guillaume J
• Carey, Lucas B
• Kondrashov, Fyodor A
• Petrov, Dmitri A.

Titel

An experimental assay of the interactions of amino acids from orthologous sequences shaping a complex fitness landscape

Ist Teil von

• PLoS genetics, 2019-04, Vol.15 (4), p.e1008079

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2019

Quelle

MEDLINE

Beschreibungen/Notizen

• Characterizing the fitness landscape, a representation of fitness for a large set of genotypes, is key to understanding how genetic information is interpreted to create functional organisms. Here we determined the evolutionarily-relevant segment of the fitness landscape of His3, a gene coding for an enzyme in the histidine synthesis pathway, focusing on combinations of amino acid states found at orthologous sites of extant species. Just 15% of amino acids found in yeast His3 orthologues were always neutral while the impact on fitness of the remaining 85% depended on the genetic background. Furthermore, at 67% of sites, amino acid replacements were under sign epistasis, having both strongly positive and negative effect in different genetic backgrounds. 46% of sites were under reciprocal sign epistasis. The fitness impact of amino acid replacements was influenced by only a few genetic backgrounds but involved interaction of multiple sites, shaping a rugged fitness landscape in which many of the shortest paths between highly fit genotypes are inaccessible.