PloS one, 2019-05, Vol.14 (5), p.e0203101-e0203101
2019
Details
- Autor(en) / Beteiligte
- Titel
- Petite Integration Factor 1 (PIF1) helicase deficiency increases weight gain in Western diet-fed female mice without increased inflammatory markers or decreased glucose clearance
- Ist Teil von
- PloS one, 2019-05, Vol.14 (5), p.e0203101-e0203101
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Petite Integration Factor 1 (PIF1) is a multifunctional helicase present in nuclei and mitochondria. PIF1 knock out (KO) mice exhibit accelerated weight gain and decreased wheel running on a normal chow diet. In the current study, we investigated whether Pif1 ablation alters whole body metabolism in response to weight gain. PIF1 KO and wild type (WT) C57BL/6J mice were fed a Western diet (WD) rich in fat and carbohydrates before evaluation of their metabolic phenotype. Compared with weight gain-resistant WT female mice, WD-fed PIF1 KO females, but not males, showed accelerated adipose deposition, decreased locomotor activity, and reduced whole-body energy expenditure without increased dietary intake. Surprisingly, PIF1 KO females did not show obesity-induced alterations in fasting blood glucose and glucose clearance. WD-fed PIF1 KO females developed mild hepatic steatosis and associated changes in liver gene expression that were absent in weight-matched, WD-fed female controls, linking hepatic steatosis to Pif1 ablation rather than increased body weight. WD-fed PIF1 KO females also showed decreased expression of inflammation-associated genes in adipose tissue. Collectively, these data separated weight gain from inflammation and impaired glucose homeostasis. They also support a role for Pif1 in weight gain resistance and liver metabolic dysregulation during nutrient stress.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0203101Titel-ID: cdi_plos_journals_2231406628
- Format
- –
- Schlagworte
- Ablation, Adipose tissue, Adipose Tissue - metabolism, Analysis, Animals, Biology and Life Sciences, Blood glucose, Body Composition, Body weight, Body weight gain, Carbohydrates, Cardiology, Care and treatment, Cell cycle, Cholesterol - metabolism, Critical care, Cytokines - metabolism, Dextrose, Diabetes, Diet, Diet, Western, Dietary intake, DNA helicase, DNA Helicases - deficiency, EDTA, Endocrinology, Endocrinology and metabolism, Energy expenditure, Energy Metabolism, Fasting, Fatty liver, Fatty Liver - metabolism, Fatty Liver - pathology, Female, Females, Food and Nutrition, Gender differences, Gene expression, Genes, Genetics, Glucose, Glucose - metabolism, Glucose Tolerance Test, Health aspects, Health care, Homeostasis, House mouse, Human health and pathology, Inflammation, Inflammation Mediators - metabolism, Insulin resistance, Integration, Laboratory animals, Life Sciences, Liver, Liver - metabolism, Liver - pathology, Locomotor activity, Male, Males, Medicine, Medicine and Health Sciences, Metabolic syndrome, Metabolism, Mice, Mice, Knockout, Mitochondria, Mitochondria - metabolism, Motor Activity, Nutrition research, Obesity, Pathogenesis, Phenotypes, Physiology, Rodents, Steatosis, Telomerase, Type 2 diabetes, Weight gain, Weight Gain - genetics, Wheel running