PloS one, 2019-03, Vol.14 (3), p.e0213744-e0213744
2019
Details
- Autor(en) / Beteiligte
- Titel
- Analysis of the dysregulation between regulatory B and T cells (Breg and Treg) in human immunodeficiency virus (HIV)-infected patients
- Ist Teil von
- PloS one, 2019-03, Vol.14 (3), p.e0213744-e0213744
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- This study examines the relationship between regulatory B (Breg) and T (Treg) compartments, which play crucial roles in the maintenance of immune homeostasis in the context of HIV. Using flow cytometry, the phenotypes of different Breg and Treg subsets from HIV-infected and healthy individuals were analyzed, along with the suppressive capacity of Breg. Peripheral blood samples of thirteen HIV+ treatment-naïve individuals, fourteen treated-HIV+ individuals with undetectable viral load and twelve healthy individuals were analyzed. The absolute counts of Breg and Treg subsets were decreased in HIV+ treatment-naïve individuals in comparison to treated-HIV+ and healthy individuals. Interestingly, correlations between Breg subsets (CD24hiCD27+ and PD-L1+ B cells) and IL-10-producing Breg observed in healthy individuals were lost in HIV+ treatment-naïve individuals. However, a correlation between frequencies of CD24hiCD38hi or TIM-1+-Breg subsets and Treg was observed in HIV+ treatment-naïve individuals and not in healthy individuals. Therefore, we hypothesized that various Breg subsets might have different functions during B and T-cell homeostasis during HIV-1 infection. In parallel, stimulated Breg from HIV-infected treatment-naïve individuals presented a decreased ability to suppress CD4+ T-cell proliferation in comparison to the stimulated Breg from treated-HIV+ or healthy individuals. We demonstrate a dysregulation between Breg and Treg subsets in HIV-infected individuals, which might participate in the hyper-activation and exhaustion of the immune system that occurs in such patients.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0213744Titel-ID: cdi_plos_journals_2199323167
- Format
- –
- Schlagworte
- Adult, Analysis, Arthritis, B-Lymphocyte Subsets - cytology, B-Lymphocyte Subsets - immunology, B-Lymphocyte Subsets - metabolism, B-Lymphocytes, Regulatory - cytology, B-Lymphocytes, Regulatory - immunology, B-Lymphocytes, Regulatory - metabolism, Biology and Life Sciences, Breast cancer, Cardiovascular disease, Case-Control Studies, CD4 antigen, Cell Proliferation, Correlation, Dendritic cells, Exhaustion, Female, Flow cytometry, Health aspects, HIV, HIV (Viruses), HIV - isolation & purification, HIV - physiology, HIV Infections - immunology, HIV Infections - pathology, HIV patients, Homeostasis, Hospitals, Human immunodeficiency virus, Humans, Immune system, Infections, Infectious diseases, Inflammation, Interleukin 10, Interleukin-10 - metabolism, Laboratories, Leukocytes, Mononuclear - cytology, Leukocytes, Mononuclear - metabolism, Ligands, Lymphocytes, Lymphocytes B, Lymphocytes T, Male, Medical research, Medicine, Medicine and Health Sciences, Middle Aged, Patients, PD-L1 protein, Peripheral blood, Phenotype, Phenotypes, Research and Analysis Methods, Surgery, T cells, T-Lymphocytes, Regulatory - cytology, T-Lymphocytes, Regulatory - immunology, T-Lymphocytes, Regulatory - metabolism, Viral Load, Viruses