PloS one, 2019-03, Vol.14 (3), p.e0213874-e0213874
2019
Details
- Autor(en) / Beteiligte
- Titel
- Effect of nitrogen supply on nitrogen metabolism in the citrus cultivar 'Huangguogan'
- Ist Teil von
- PloS one, 2019-03, Vol.14 (3), p.e0213874-e0213874
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Nitrogen metabolism in citrus has received increased attention due to its effects on plant growth and productivity. However, little is known about the effects of nitrogen fertilization on nitrogen metabolism in young trees of citrus cultivar 'Huangguogan' (Citrus reticulata × Citrus sinensis). Here, genes encoding nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate dehydrogenase (GDH), and asparagine synthetase (AS), represented as HgNR, HgNiR, HgGS, HgGDH, and HgAS, respectively, were cloned from Huangguogan. Deduced protein sequences were analyzed and proteins were confirmed to be localized in their respective cellular organelles. Moreover, pot-cultured 'Huangguogan' seedlings were fertilized with 0 (N1), 1.36 (N2), 1.81 (N3), 2.26 (N4), or 2.72 (N5) kg N/year, for 12 months. Enzyme activity and enzyme-gene expression were studied in roots, leaves, and fruits at different stages. Finally, the effects of N application rate on root activity, leaf N, soluble protein, yield, and fruit quality at the ripening stage were measured. The results showed that: 1) HgNR, HgNiR, HgGDH, and HgAS gene products were found mainly in the cytoplasm and plasma membrane, while HgGS gene product was found mainly in cytoplasm and mitochondria. 2) Gene expression and enzyme activity differed among plant organs. As the root is in permanent direct contact with the soil we suggest that root gene expression and enzyme activity can be used as reference to determine N application rate. 3) Yield, fruit quality, enzyme activity, and enzyme-related gene expression were considerably lower at low than at high-N supply. However, they were all inhibited by excess nitrogen (i.e., 2.72 kg/year). Therefore, we recommend 1.81 kg N/year as the optimal N application rate for young 'Huangguogan' trees.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0213874Titel-ID: cdi_plos_journals_2195911558
- Format
- –
- Schlagworte
- Agricultural production, Amino acids, Analysis, Asparagine, Aspartate-ammonia ligase, Biological products, Biology and Life Sciences, Cell membranes, Citrus, Citrus - growth & development, Citrus - metabolism, Citrus fruits, Citrus reticulata, Citrus sinensis, Cultivars, Cytoplasm, Cytoplasm - metabolism, Enzymatic activity, Enzyme activity, Enzymes, Fertilization, Fertilizers, Flowers & plants, Fruit - metabolism, Fruits, Gene expression, Gene Expression Regulation, Plant, Genes, Glutamate, Glutamate dehydrogenase, Glutamate Dehydrogenase - classification, Glutamate Dehydrogenase - genetics, Glutamate Dehydrogenase - metabolism, Glutamate-ammonia ligase, Glutamate-Ammonia Ligase - classification, Glutamate-Ammonia Ligase - genetics, Glutamate-Ammonia Ligase - metabolism, Glutamine, Growth, Growth (Plants), Horticulture, Leaves, Ligases, Medicine and Health Sciences, Mitochondria, Nitrate reductase, Nitrate Reductase - classification, Nitrate Reductase - genetics, Nitrate Reductase - metabolism, Nitrates, Nitrite reductase, Nitrogen, Nitrogen (Nutrient), Nitrogen - metabolism, Nitrogen in the body, Nutrition, Organelles, Organs, Phylogeny, Physical Sciences, Physiological aspects, Plant growth, Plant Leaves - metabolism, Plant metabolism, Plant Proteins - classification, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Roots - metabolism, Production management, Proteins, Ripening, Seedlings, Seedlings - growth & development, Seedlings - metabolism, Trees