PloS one, 2019-02, Vol.14 (2), p.e0212299-e0212299
2019
Details
- Autor(en) / Beteiligte
- Titel
- S100A4 inhibits cell proliferation by interfering with the S100A1-RAGE V domain
- Ist Teil von
- PloS one, 2019-02, Vol.14 (2), p.e0212299-e0212299
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The Ca2+-dependent human S100A4 (Mts1) protein is part of the S100 family. Here, we studied the interactions of S100A4 with S100A1 using nuclear magnetic resonance (NMR) spectroscopy. We used the chemical shift perturbed residues from HSQC to model S100A4 and S100A1 complex with HADDOCK software. We observed that S100A1 and the RAGE V domain have an analogous binding area in S100A4. We discovered that S100A4 acts as an antagonist among the RAGE V domain and S100A1, which inhibits tumorigenesis and cell proliferation. We used a WST-1 assay to examine the bioactivity of S100A1 and S100A4. This study could possibly be beneficial for evaluating new proteins for the treatment of diseases.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0212299Titel-ID: cdi_plos_journals_2184238903
- Format
- –
- Schlagworte
- Analysis, Angiogenesis, Apoptosis, Binding Sites, Biological activity, Biology and Life Sciences, Breasts, Calcium, Calcium ions, Cell growth, Cell Line, Tumor, Cell Proliferation, Chemical equilibrium, Chemistry, Colorectal cancer, Diagnostic imaging, Haddock, Homeopathy, Humans, Kinetics, Magnetic resonance, Materia medica and therapeutics, Medical treatment, Medicine and Health Sciences, Melanoma, Metastasis, Molecular Docking Simulation, NMR, Nuclear magnetic resonance, Nuclear magnetic resonance spectroscopy, Nuclear Magnetic Resonance, Biomolecular, Organic chemistry, Physical Sciences, Protein Binding, Proteins, Research and analysis methods, S100 Calcium-Binding Protein A4 - chemistry, S100 Calcium-Binding Protein A4 - genetics, S100 Calcium-Binding Protein A4 - metabolism, S100 Proteins - chemistry, S100 Proteins - genetics, S100 Proteins - metabolism, S100A4 protein, Spectroscopy, Therapeutics, Thyroid gland, Tumorigenesis, Tumors