PloS one, 2019-01, Vol.14 (1), p.e0210515-e0210515
2019
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Details
- Autor(en) / Beteiligte
- Titel
- FUSE binding protein 1 (FUBP1) expression is upregulated by T-cell acute lymphocytic leukemia protein 1 (TAL1) and required for efficient erythroid differentiation
- Ist Teil von
- PloS one, 2019-01, Vol.14 (1), p.e0210515-e0210515
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- During erythropoiesis, haematopoietic stem cells (HSCs) differentiate in successive steps of commitment and specification to mature erythrocytes. This differentiation process is controlled by transcription factors that establish stage- and cell type-specific gene expression. In this study, we demonstrate that FUSE binding protein 1 (FUBP1), a transcriptional regulator important for HSC self-renewal and survival, is regulated by T-cell acute lymphocytic leukaemia 1 (TAL1) in erythroid progenitor cells. TAL1 directly activates the FUBP1 promoter, leading to increased FUBP1 expression during erythroid differentiation. The binding of TAL1 to the FUBP1 promoter is highly dependent on an intact GATA sequence in a combined E-box/GATA motif. We found that FUBP1 expression is required for efficient erythropoiesis, as FUBP1-deficient progenitor cells were limited in their potential of erythroid differentiation. Thus, the finding of an interconnection between GATA1/TAL1 and FUBP1 reveals a molecular mechanism that is part of the switch from progenitor- to erythrocyte-specific gene expression. In summary, we identified a TAL1/FUBP1 transcriptional relationship, whose physiological function in haematopoiesis is connected to proper erythropoiesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0210515Titel-ID: cdi_plos_journals_2168181295
- Format
- –
- Schlagworte
- A549 Cells, Acute lymphoblastic leukemia, Adapter proteins, Apoptosis, Binding sites, Biology, Biology and Life Sciences, Cell cycle, Cell Differentiation - genetics, Cell self-renewal, Cell survival, Cells (biology), Differentiation, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Erythrocytes, Erythroid Precursor Cells - metabolism, Erythropoiesis, Erythropoiesis - genetics, GATA-1 protein, GATA1 Transcription Factor - genetics, GATA1 Transcription Factor - metabolism, Gene expression, Gene Expression Regulation, Neoplastic, HEK293 Cells, Hematopoietic stem cells, HL-60 Cells, Humans, Leukemia, Lymphatic leukemia, Lymphocytes T, Medicine, Medicine and Health Sciences, Progenitor cells, Proteins, Research and Analysis Methods, RNA-Binding Proteins - genetics, RNA-Binding Proteins - metabolism, Stem cells, T-Cell Acute Lymphocytic Leukemia Protein 1 - genetics, T-Cell Acute Lymphocytic Leukemia Protein 1 - metabolism, Transcription factors, Up-Regulation