PloS one, 2018-11, Vol.13 (11), p.e0207405-e0207405
2018
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- Autor(en) / Beteiligte
- Titel
- Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer
- Ist Teil von
- PloS one, 2018-11, Vol.13 (11), p.e0207405-e0207405
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2018
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Modulation or prevention of protein changes during the cholangiocarcinoma (CCA) process induced by Opisthorchis viverrini (Ov) infection may become a key strategy for prevention and treatment of CCA. Monitoring of such changes could lead to discovery of protein targets for CCA treatment. Curcumin exerts anti-inflammatory and anti-CCA activities partly through its protein-modulatory ability. To support the potential use of curcumin and to discover novel target molecules for CCA treatment, we used a quantitative proteomic approach to investigate the effects of curcumin on protein changes in an Ov-induced CCA-harboring hamster model. Isobaric labelling and tandem mass spectrometry were used to compare the protein expression profiles of liver tissues from CCA hamsters with or without curcumin dietary supplementation. Among the dysregulated proteins, five were upregulated in liver tissues of CCA hamsters but markedly downregulated in the CCA hamsters supplemented with curcumin: S100A6, lumican, plastin-2, 14-3-3 zeta/delta and vimentin. Western blot and immunohistochemical analyses also showed similar expression patterns of these proteins in liver tissues of hamsters in the CCA and CCA + curcumin groups. Proteins such as clusterin and S100A10, involved in the NF-κB signaling pathway, an important signaling cascade involved in CCA genesis, were also upregulated in CCA hamsters and were then suppressed by curcumin treatment. Taken together, our results demonstrate the important changes in the proteome during the genesis of O. viverrini-induced CCA and provide an insight into the possible protein targets for prevention and treatment of this cancer.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0207405Titel-ID: cdi_plos_journals_2133833854
- Format
- –
- Schlagworte
- 14-3-3 protein, 14-3-3 Proteins - genetics, Animals, Bile Duct Neoplasms - complications, Bile Duct Neoplasms - drug therapy, Bile Duct Neoplasms - pathology, Bile Duct Neoplasms - prevention & control, Bile ducts, Biology and Life Sciences, Biomarkers, Calcium-binding protein, Cancer, Cancer therapies, Chemoprevention, Chemotherapy, Cholangiocarcinoma, Cholangiocarcinoma - complications, Cholangiocarcinoma - drug therapy, Cholangiocarcinoma - genetics, Cholangiocarcinoma - pathology, Clusterin, Cricetinae, Curcumin, Curcumin - administration & dosage, Cytochrome, Diet, Dietary supplements, Disease Models, Animal, Fasciola hepatica - drug effects, Gene Expression Regulation - drug effects, Hamsters, Health sciences, Humans, Immunology, Infections, Inflammation, Kinases, Labelling, Liver, Liver - drug effects, Liver - pathology, Lumican - genetics, Mass spectrometry, Mass spectroscopy, Medicine, Medicine and Health Sciences, Membrane Glycoproteins - genetics, Microfilament Proteins - genetics, NF-κB protein, Opisthorchiasis - complications, Opisthorchiasis - drug therapy, Opisthorchiasis - genetics, Opisthorchiasis - pathology, Opisthorchis - pathogenicity, Opisthorchis viverrini, Parasitology, Prevention, Proteins, Proteomes, Proteomics, R&D, Research & development, Research and Analysis Methods, S100 Calcium Binding Protein A6 - genetics, S100 protein, Signal transduction, Signaling, Tissues, Tumorigenesis, Vimentin, Vimentin - genetics