Details

Autor(en) / Beteiligte

• Medeiros, Fernanda Silva
• Martins, Albert Eduardo Silva
• Gomes, Renan Garcia
• de Oliveira, Sávio Augusto Vieira
• Welkovic, Stefan
• Maruza, Magda
• Menezes, Maria Luiza Bezerra
• Ximenes, Ricardo Arraes de Alencar
• Diniz, George Tadeu Nunes
• Donadi, Eduardo Antônio
• Lucena-Silva, Norma
• Tornesello, Maria Lina

Titel

Variation sites at the HLA-G 3' untranslated region confer differential susceptibility to HIV/HPV co-infection and aneuploidy in cervical cell

Ist Teil von

• PloS one, 2018-10, Vol.13 (10), p.e0204679-e0204679

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2018

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Post-transcriptional regulatory elements associated with transcript degradation or transcript instability have been described at the 3' untranslated region (3'UTR) of the HLA-G gene. Considering that HPV infection and aneuploidy, which causes gene instability, are associated with cervical cell malignancy, as well as the fact that HIV infection and HLA-G may modulate the immune response, the present study aimed to compare the frequencies of HLA-G 3'UTR polymorphic sites (14-base pair insertion/deletion, +3142C/G, and +3187A/G) between 226 HIV+ women co-infected (n = 82) or not with HPV (n = 144) and 138 healthy women. We also evaluated the relationship between those HLA-G 3'UTR variants and aneuploidy in cervical cells. HPV types and HLA-G polymorphisms were determined by PCR and sequencing of cervical samples DNA. Aneuploidy in cervical cell was measured by flow cytometry. The HLA-G 3'UTR 14-bp ins/del was not associated with either HIV nor HIV/HPV co-infection. The +3142G allele (p = 0.049) and +3142GG genotype (p = 0.047) were overrepresented in all HIV-infected women. On the other hand, the +3187G allele (p = 0.028) and the +3187GG genotype (p = 0.026) predominated among healthy women. The +3142G (p = 0.023) and +3187A (p = 0.003) alleles were associated with predisposition to HIV infection, irrespective of the presence or not of HIV/HPV co-infection. The diplotype formed by the combination of the +3142CX (CC or CG) and +3187AA genotype conferred the highest risk for aneuploidy in cervical cell induced by HPV. The HLA-G 3'UTR +3142 and +3187 variants conferred distinct susceptibility to HIV infection and aneuploidy.