PloS one, 2018-09, Vol.13 (9), p.e0202590-e0202590
2018
Details
- Autor(en) / Beteiligte
- Titel
- Therapeutic potential of extracellular vesicles derived from human mesenchymal stem cells in a model of progressive multiple sclerosis
- Ist Teil von
- PloS one, 2018-09, Vol.13 (9), p.e0202590-e0202590
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication and as possible therapeutic agents in inflammation-mediated demyelinating diseases, including multiple sclerosis (MS). In the present study, we investigated whether intravenously administered EVs derived from mesenchymal stem cells (MSCs) from human adipose tissue might mediate recovery in Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease, a progressive model of MS. SJL/J mice were subjected to EV treatment once the disease was established. We found that intravenous EV administration improved motor deficits, reduced brain atrophy, increased cell proliferation in the subventricular zone and decreased inflammatory infiltrates in the spinal cord in mice infected with TMEV. EV treatment was also capable of modulating neuroinflammation, given glial fibrillary acidic protein and Iba-1 staining were reduced in the brain, whereas myelin protein expression was increased. Changes in the morphology of microglial cells in the spinal cord suggest that EVs also modulate the activation state of microglia. The clear reduction in plasma cytokine levels, mainly in the Th1 and Th17 phenotypes, in TMEV mice treated with EVs confirms the immunomodulatory ability of intravenous EVs. According to our results, EV administration attenuates motor deficits through immunomodulatory actions, diminishing brain atrophy and promoting remyelination. Further studies are necessary to establish EV delivery as a possible therapy for the neurodegenerative phase of MS.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0202590Titel-ID: cdi_plos_journals_2110053398
- Format
- –
- Schlagworte
- Adipose tissue, Adipose Tissue - cytology, Adipose tissues, Administration, Intravenous, Animals, Atrophy, Biology and Life Sciences, Brain, Brain research, Calcium-Binding Proteins - metabolism, Care and treatment, Cell Proliferation, Cell signaling, Chemical compounds, Demyelinating diseases, Demyelination, Disease, Disease Models, Animal, Encephalomyelitis, Extracellular vesicles, Extracellular Vesicles - transplantation, Gene Expression Regulation, Glial fibrillary acidic protein, Glial Fibrillary Acidic Protein - metabolism, Health aspects, Helper cells, Hospitals, Humans, Immunomodulation, Inflammation, Intravenous administration, Laboratories, Lymphocytes T, Medical research, Medical treatment, Medicine and Health Sciences, Mesenchymal stem cells, Mesenchymal Stem Cells - cytology, Mesenchyme, Mice, Microfilament Proteins - metabolism, Microglia, Microglial cells, Morphology, Motors, Multiple sclerosis, Multiple Sclerosis - immunology, Multiple Sclerosis - therapy, Multiple Sclerosis - virology, Myelin, Myelin Proteins - metabolism, Myelination, Nervous system, Neurobiology, Neurodegeneration, Neurology, Neuronal-glial interactions, Neurosciences, Pharmacology, Phenotypes, Proteins, Research and Analysis Methods, Spinal cord, Stem cells, Stroke, Theilovirus - pathogenicity, Treatment Outcome, Vesicles, Viruses