Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor
2018
Details
- Autor(en) / Beteiligte
- Titel
- Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor
- Ist Teil von
- PLoS pathogens, 2018-03, Vol.14 (3), p.e1006914-e1006914
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Macrophages are a diverse group of phagocytic cells acting in host protection against stress, injury, and pathogens. Here, we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells, and important for production of type I interferon. Scavenger receptors contribute to the clearance of endogenous proteins, lipoproteins and pathogens. Knockout of SR-A6 in MPI cells, anti-SR-A6 antibody or the soluble extracellular SR-A6 domain reduced adenovirus type-C5 (HAdV-C5) binding and transduction. Expression of murine SR-A6, and to a lower extent human SR-A6 boosted virion binding to human cells and transduction. Virion clustering by soluble SR-A6 and proximity localization with SR-A6 on MPI cells suggested direct adenovirus interaction with SR-A6. Deletion of the negatively charged hypervariable region 1 (HVR1) of hexon reduced HAdV-C5 binding and transduction, implying that the viral ligand for SR-A6 is hexon. SR-A6 facilitated macrophage entry of HAdV-B35 and HAdV-D26, two important vectors for transduction of hematopoietic cells and human vaccination. The study highlights the importance of scavenger receptors in innate immunity against human viruses.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7374, 1553-7366eISSN: 1553-7374DOI: 10.1371/journal.ppat.1006914Titel-ID: cdi_plos_journals_2025704013
- Format
- –
- Schlagworte
- Adenoviridae Infections - immunology, Adenoviridae Infections - metabolism, Adenoviridae Infections - virology, Adenoviruses, Adenoviruses, Human - immunology, Alveoli, Animals, Binding, Binding sites, Biochemistry, Biology and Life Sciences, Clonal deletion, Clustering, Epigenetics, Grants, Humans, Immunity, Immunity, Innate, Infections, Innate immunity, Interferon, Laboratory animals, Life sciences, Lipoproteins, Localization, Lung - immunology, Lung - metabolism, Lung - virology, Lungs, Macrophages, Macrophages - immunology, Macrophages - metabolism, Macrophages - virology, Macrophages, Alveolar - immunology, Macrophages, Alveolar - metabolism, Macrophages, Alveolar - virology, MARCO protein, Medicine, Medicine and Health Sciences, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Pathogens, Phagocytes, Protein Binding, Proteins, Receptors, Receptors, Immunologic - genetics, Receptors, Immunologic - metabolism, Receptors, Immunologic - physiology, Research and Analysis Methods, Scavenger receptors, Thoracic surgery, Vaccination, Vectors, Virions, Virus Internalization, Viruses