Details
- Autor(en) / Beteiligte
- Titel
- The activated SA and JA signaling pathways have an influence on flg22-triggered oxidative burst and callose deposition
- Ist Teil von
- PloS one, 2014-02, Vol.9 (2), p.e88951-e88951
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- The first line of defense in plants against pathogens is induced by the recognition of microbe-associated molecular patterns (MAMP). Perception of bacterial flagellin (flg22) by the pattern recognition receptor flagellin-sensing 2 (FLS2) is the best characterized MAMP response, although the underlying molecular mechanisms are not fully understood. Here we studied the relationship between salicylic acid (SA) or jasmonic acid (JA) signaling and FLS2-mediated signaling by monitoring flg22-triggered responses in known SA or JA related mutants of Arabidopsis thaliana (L.) Heynh. The sid2 mutant, impaired in SA biosynthesis, had less basal FLS2 mRNA accumulation than the wild type, which correlated with suppression of early flg22 responses such as ROS production and induction of marker genes, WRKY29 and FRK1. The JA-signaling mutants, jar1 and coi1, exhibited an enhanced flg22-triggered oxidative burst and more callose accumulation than the wild type, and pretreatment with SA or coronatine (COR), a structural mimic of JA-isoleucine, altered these flg22-induced responses. Nonexpressor of pathogenesis-related genes 1 (NPR1) acted downstream of SID2 and required SA-dependent priming for the enhanced flg22-triggered oxidative burst and callose deposition. Activation of JA signaling by COR pretreatment suppressed the flg22-triggered oxidative burst and callose accumulation in a coronatine insensitive 1 (COI1) dependent manner. COR had a negative effect on flg22 responses but only the flg22-triggered oxidative burst depended on SA-JA/COR signaling antagonism. Thus the activated SA and JA signaling pathways have an influence on flg22-triggered oxidative burst and callose deposition. These results may explain how SA and JA signaling are cross talked for regulation of flg22-triggered responses.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0088951Titel-ID: cdi_plos_journals_1977559112
- Format
- –
- Schlagworte
- Accumulation, Acids, Arabidopsis, Arabidopsis - genetics, Arabidopsis - metabolism, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Arabidopsis thaliana, Bacteria, Biology, Biosynthesis, Bursting, Coronatine, Cyclopentanes - metabolism, Defense industry, Deposition, Engineering research, Enzymes, Flagellin, Flagellin - genetics, Flagellin - metabolism, Gene expression, Gene Expression Regulation, Plant - genetics, Genes, Genetic engineering, Glucans - genetics, Glucans - metabolism, Intramolecular Transferases - genetics, Intramolecular Transferases - metabolism, Isoleucine, Jasmonic acid, Kinases, Molecular modelling, mRNA, Mutants, Mutation - genetics, Nucleotidyltransferases - genetics, Nucleotidyltransferases - metabolism, Oxylipins - metabolism, Pathogenesis, Pathogens, Pathways, Pattern recognition, Physiological aspects, Plants (botany), Priming, Protein Kinases - genetics, Protein Kinases - metabolism, Proteins, Pseudomonas syringae, Reactive oxygen species, Reactive Oxygen Species - metabolism, Respiratory Burst - genetics, RNA, Messenger - genetics, Salicylic acid, Salicylic Acid - metabolism, Signal monitoring, Signal transduction, Signal Transduction - genetics, Signaling, Transcription factors