PloS one, 2017-11, Vol.12 (11), p.e0188289
2017
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- Autor(en) / Beteiligte
- Titel
- Sulfur dioxide alleviates programmed cell death in barley aleurone by acting as an antioxidant
- Ist Teil von
- PloS one, 2017-11, Vol.12 (11), p.e0188289
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Sulfur dioxide (SO2), a gaseous signaling molecule in animal cells, has recently been found to play a physiological role in plants. Here we studied the role of SO2 in gibberellic acid (GA3)-induced programmed cell death (PCD) in barley (Hordeum vulgare L.) aleurone layers. The application of the SO2 donor (NaHSO3/Na2SO3, 1:3 M/M) effectively alleviated PCD in barley aleurone layers in a dose-dependent manner with an optimal concentration of 50 μM. Further investigations showed that SO2 reduced the accumulation of hydrogen peroxide (H2O2), superoxide anion (⋅O2-) and malondialdehyde (MDA) in aleurone layers. Moreover, the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR) and guaiacol peroxidase (POD) were enhanced by SO2 donor treatment. Meanwhile, lipoxygenase (LOX) activity was attenuated by SO2 donor treatment. Furthermore, an induction of endogenous H2S and NO were also observed in SO2-treated aleurone layers, suggesting interactions of SO2 with other well-known signaling molecules. Taken together, we show that SO2 negatively regulated PCD by acting as an antioxidant to scavenge excessive reactive oxygen species (ROS) generated during PCD.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0188289Titel-ID: cdi_plos_journals_1966424725
- Format
- –
- Schlagworte
- Aluminum, Antioxidants, Antioxidants - metabolism, Antioxidants - pharmacology, Apoptosis, Apoptosis - drug effects, Ascorbate Peroxidases - metabolism, Ascorbic acid, Barley, Biology and Life Sciences, Catalase, Catalase - metabolism, Cell death, Engineering, Enzymes, Food science, Gibberellic acid, Gibberellins - antagonists & inhibitors, Gibberellins - pharmacology, Glutathione, Glutathione reductase, Glutathione Reductase - metabolism, Guaiacol, Hordeum - drug effects, Hordeum - metabolism, Hydrogen, Hydrogen peroxide, Hydrogen sulfide, L-Ascorbate peroxidase, Lipoxygenase, Lipoxygenase - metabolism, Liquid oxygen, Malondialdehyde, Malondialdehyde - metabolism, Metabolism, Methods, Oxidative stress, Oxygen, Peroxidase, Peroxidase - metabolism, Physical Sciences, Physiological aspects, Physiology, Plant proteins, Plant Proteins - metabolism, Reactive oxygen species, Research and Analysis Methods, Seeds, Signal Transduction, Signaling, Sulfates - chemistry, Sulfur, Sulfur dioxide, Sulfur Dioxide - metabolism, Sulfur Dioxide - pharmacology, Superoxide dismutase, Superoxide Dismutase - metabolism, Superoxides - metabolism