PloS one, 2017-10, Vol.12 (10), p.e0186138-e0186138
2017
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Details
- Autor(en) / Beteiligte
- Titel
- Therapeutic effect of imiquimod on dextran sulfate sodium-induced ulcerative colitis in mice
- Ist Teil von
- PloS one, 2017-10, Vol.12 (10), p.e0186138-e0186138
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Imiquimod is a Toll-like receptor-7 agonist that regulates immunity and can be used as an immune adjuvant. Ulcerative colitis has a close correlation with immune disorder. To investigate the therapeutic effect of imiquimod on dextran sulfate sodium (DSS)-induced colitis and explore the underlying mechanisms. C57BL/6J C57 mice received 3% DSS for 7 days to induce ulcerative colitis. Groups of mice were intraperitoneally injected with dexamethasone (DXM, 1.5 mg/kg) or imiquimod (IMQ, 30 mg/kg) at the same time daily. During the experimental period, clinical signs, body weight, stool consistency and visible fecal blood were monitored and recorded daily; colitis was evaluated by disease activity index (DAI) score and by histological score. At the conclusion of the experiment, the level of colonic myeloperoxidase (MPO) activity and the serum levels of the cytokines tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6) and interleukin 10 (IL-10) were measured. Administration of 3% DSS for 7 days successfully induced acute colitis associated with diarrhea, bloody mucopurulent stool, body weight decreases, and other changes. Colitis severity was significantly ameliorated in the IMQ treatment groups, as determined by hematoxylin-eosin (HE) staining and histopathological scores. Moreover, IMQ significantly reduced the activity of MPO in colonic tissue and the serum levels of inflammatory cytokines, increased colon length and spleen weight, and effectively inhibited microscopic damage to the colon tissue. IMQ had beneficial effects on DSS-induced ulcerative colitis, supporting its further development and clinical application in ulcerative colitis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0186138Titel-ID: cdi_plos_journals_1953157464
- Format
- –
- Schlagworte
- Adjuvants, Immunologic - therapeutic use, Aminoquinolines - therapeutic use, Animals, Antiviral drugs, Biology and Life Sciences, Body weight, Carbon monoxide, Colitis, Ulcerative - chemically induced, Colitis, Ulcerative - drug therapy, Colon, Colon - drug effects, Colon - enzymology, Colon - pathology, Complications and side effects, Cytokines, Cytokines - biosynthesis, Dendritic cells, Dexamethasone, Dextran, Dextran sulfate, Dextran Sulfate - adverse effects, Diarrhea, Digestive system, Dosage and administration, Drug therapy, Gastroenterology, Health aspects, Homeostasis, Imiquimod, Immune system, Immunity, Inflammatory bowel disease, Inflammatory bowel diseases, Interleukin 10, Interleukin 6, Laboratory animals, Male, Medicine and Health Sciences, Mice, Mice, Inbred C57BL, Pathogenesis, Peroxidase, Peroxidase - metabolism, Proteins, Rodents, Serum levels, Sodium, Spleen, Sulfates, Toll-like receptors, Tumor necrosis factor-α, Ulcerative colitis