Details
- Autor(en) / Beteiligte
- Titel
- The Pafah1b complex interacts with the reelin receptor VLDLR
- Ist Teil von
- PloS one, 2007-02, Vol.2 (2), p.e252-e252
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2007
- Link zum Volltext
- Quelle
- Electronic Journals Library - Freely accessible e-journals
- Beschreibungen/Notizen
- Reelin is an extracellular protein that directs the organization of cortical structures of the brain through the activation of two receptors, the very low-density lipoprotein receptor (VLDLR) and the apolipoprotein E receptor 2 (ApoER2), and the phosphorylation of Disabled-1 (Dab1). Lis1, the product of the Pafah1b1 gene, is a component of the brain platelet-activating factor acetylhydrolase 1b (Pafah1b) complex, and binds to phosphorylated Dab1 in response to Reelin. Here we investigated the involvement of the whole Pafah1b complex in Reelin signaling and cortical layer formation and found that catalytic subunits of the Pafah1b complex, Pafah1b2 and Pafah1b3, specifically bind to the NPxYL sequence of VLDLR, but not to ApoER2. Compound Pafah1b1(+/-);Apoer2(-/-) mutant mice exhibit a reeler-like phenotype in the forebrain consisting of the inversion of cortical layers and hippocampal disorganization, whereas double Pafah1b1(+/-);Vldlr(-/-) mutants do not. These results suggest that a cross-talk between the Pafah1b complex and Reelin occurs downstream of the VLDLR receptor.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0000252Titel-ID: cdi_plos_journals_1950102769
- Format
- –
- Schlagworte
- 1-Alkyl-2-acetylglycerophosphocholine Esterase - deficiency, 1-Alkyl-2-acetylglycerophosphocholine Esterase - genetics, 1-Alkyl-2-acetylglycerophosphocholine Esterase - metabolism, Acetylcholinesterase, Adapter proteins, Amino Acid Motifs, Amino Acid Sequence, Animals, Apolipoprotein E, Ataxia, Biochemistry/Cell Signaling and Trafficking Structures, Brain, Catalysis, Catalytic Domain, Catalytic subunits, Cell Adhesion Molecules, Neuronal - physiology, Cell Biology/Cell Signaling, Cell Biology/Developmental Molecular Mechanisms, Cell Biology/Neuronal and Glial Cell Biology, Cell Biology/Neuronal Signaling Mechanisms, Cell Line, Cercopithecus aethiops, Cerebral Cortex - abnormalities, Children & youth, Cortex, COS Cells, Developmental Biology/Neurodevelopment, Disabled-1 protein, Extracellular Matrix Proteins - physiology, Forebrain, Genes, Genotype & phenotype, Hippocampus, Hippocampus - abnormalities, Humans, Immunoglobulins, Kinases, Laboratories, LDL-Receptor Related Proteins, Ligands, Lissencephaly - genetics, Lissencephaly - metabolism, Lissencephaly - pathology, Low density lipoprotein receptors, Low density lipoproteins, Medicine, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Neurologic Mutants, Microtubule-Associated Proteins - deficiency, Microtubule-Associated Proteins - genetics, Microtubule-Associated Proteins - metabolism, Molecular Sequence Data, Mutants, Mutation, Nerve Tissue Proteins - physiology, Neurological Disorders/Epilepsy, Neuroscience/Neuronal and Glial Cell Biology, Neuroscience/Neuronal Signaling Mechanisms, Neurosciences, Pediatrics, Phosphorylation, Platelet-activating factor, Platelet-activating factor acetylhydrolase, Protein Binding, Protein Interaction Mapping, Receptor density, Receptors, Receptors, Cell Surface - deficiency, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, Receptors, LDL - deficiency, Receptors, LDL - genetics, Receptors, LDL - metabolism, Receptors, LDL - physiology, Receptors, Lipoprotein - deficiency, Receptors, Lipoprotein - genetics, Receptors, Lipoprotein - metabolism, Receptors, Lipoprotein - physiology, Recombinant Fusion Proteins - metabolism, Reelin protein, Serine Endopeptidases - physiology, Signal transduction, Signal Transduction - physiology, Studies