PLoS genetics, 2017-07, Vol.13 (7), p.e1006890-e1006890
2017
Details
- Autor(en) / Beteiligte
- Titel
- Xist RNA repeat E is essential for ASH2L recruitment to the inactive X and regulates histone modifications and escape gene expression
- Ist Teil von
- PLoS genetics, 2017-07, Vol.13 (7), p.e1006890-e1006890
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Long non-coding RNA Xist plays a crucial role in establishing and maintaining X-chromosome inactivation (XCI) which is a paradigm of long non-coding RNA-mediated gene regulation. Xist has Xist-specific repeat elements A-F which are conserved among eutherian mammals, underscoring their functional importance. Here we report that Xist RNA repeat E, a conserved Xist repeat element in the Xist exon 7, interacts with ASH2L and contributes to maintenance of escape gene expression level on the inactive X-chromosome (Xi) during XCI. The Xist repeat E-deletion mutant female ES cells show the depletion of ASH2L from the Xi upon differentiation. Furthermore, a subset of escape genes exhibits unexpectedly higher expression in the repeat E mutant cells than the cells expressing wildtype Xist during X-inactivation, whereas the silencing of X-linked non-escape genes is not affected. We discuss the implications of these results to understand the role of ASH2L and Xist repeat E for histone modifications and escape gene regulation during random X-chromosome inactivation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7404, 1553-7390eISSN: 1553-7404DOI: 10.1371/journal.pgen.1006890Titel-ID: cdi_plos_journals_1929401149
- Format
- –
- Schlagworte
- Animals, Biology and Life Sciences, Breast cancer, Cell Differentiation - genetics, Chromatin, Chromosome deletion, Chromosomes, Clonal deletion, Deactivation, Deletion mutant, DNA-Binding Proteins - biosynthesis, DNA-Binding Proteins - genetics, Drug dosages, Embryonic Stem Cells - metabolism, Enzymes, Exons - genetics, Females, Funding, Gene Deletion, Gene expression, Gene Expression Regulation, Gene regulation, Gene Silencing, Genomics, Histone Code - genetics, Histones, Medical research, Medicine, Medicine and Health Sciences, Methods, Mice, Non-coding RNA, Nuclear Proteins - biosynthesis, Nuclear Proteins - genetics, Observations, Pediatrics, Physiological aspects, Research and Analysis Methods, RNA sequencing, RNA, Long Noncoding - genetics, Roles, Transcription Factors - biosynthesis, Transcription Factors - genetics, Transcription, Genetic, X Chromosome - genetics, X Chromosome Inactivation - genetics, X chromosomes, X-chromosome inactivation