PloS one, 2017-07, Vol.12 (7), p.e0180989-e0180989
2017
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- Autor(en) / Beteiligte
- Titel
- Endurance performance and energy metabolism during exercise in mice with a muscle-specific defect in the control of branched-chain amino acid catabolism
- Ist Teil von
- PloS one, 2017-07, Vol.12 (7), p.e0180989-e0180989
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- It is known that the catabolism of branched-chain amino acids (BCAAs) in skeletal muscle is suppressed under normal and sedentary conditions but is promoted by exercise. BCAA catabolism in muscle tissues is regulated by the branched-chain α-keto acid (BCKA) dehydrogenase complex, which is inactivated by phosphorylation by BCKA dehydrogenase kinase (BDK). In the present study, we used muscle-specific BDK deficient mice (BDK-mKO mice) to examine the effect of uncontrolled BCAA catabolism on endurance exercise performance and skeletal muscle energy metabolism. Untrained control and BDK-mKO mice showed the same performance; however, the endurance performance enhanced by 2 weeks of running training was somewhat, but significantly less in BDK-mKO mice than in control mice. Skeletal muscle of BDK-mKO mice had low levels of glycogen. Metabolome analysis showed that BCAA catabolism was greatly enhanced in the muscle of BDK-mKO mice and produced branched-chain acyl-carnitine, which induced perturbation of energy metabolism in the muscle. These results suggest that the tight regulation of BCAA catabolism in muscles is important for homeostasis of muscle energy metabolism and, at least in part, for adaptation to exercise training.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0180989Titel-ID: cdi_plos_journals_1919959704
- Format
- –
- Schlagworte
- Adaptation, Amino acids, Amino Acids, Branched-Chain - metabolism, Analysis, Animals, Biology and Life Sciences, Biosynthesis, Branched chain amino acids, Carnitine, Catabolism, Chain branching, Citric Acid Cycle, Defects, Dehydrogenase, Dehydrogenases, Diet, Durability, Endurance, Energy Metabolism, Enzymes, Exercise, Gene Knockout Techniques, Glycogen, Glycosylation, Health aspects, Homeostasis, Male, Medicine and Health Sciences, Metabolism, Metabolomics, Methods, Mice, Mitochondria - metabolism, Muscles, Muscles - metabolism, Muscles - physiology, Musculoskeletal system, NAD - metabolism, Organ Specificity, Oxidation-Reduction, Perturbation methods, Phosphatase, Phosphorylation, Physical Conditioning, Animal - physiology, Physical Endurance, Physical training, Physiological aspects, Physiology, Properties, Protein Kinases - deficiency, Protein Kinases - genetics, Protein Kinases - metabolism, Proteins, Rodents, Skeletal muscle, Swimming pools, Tissues, Training