Genome wide host gene expression analysis in mice experimentally infected with Pasteurella multocida
2017
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- Autor(en) / Beteiligte
- Titel
- Genome wide host gene expression analysis in mice experimentally infected with Pasteurella multocida
- Ist Teil von
- PloS one, 2017-07, Vol.12 (7), p.e0179420-e0179420
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Pasteurella multocida causes acute septicemic and respiratory diseases, including haemorrhagic septicaemia, in cattle and buffalo with case fatality of 100%. In the present study, mice were infected with P. multocida (1.6 × 103 cfu, intraperitoneal) to evaluate host gene expression profile at early and late stages of infection using high throughput microarray transcriptome analyses. Several differentially expressed genes (DEGs) at both the time points were identified in P.multocida infected spleen, liver and lungs. Functional annotation of these DEGs showed enrichment of key pathways such as TLR, NF-κB, MAPK, TNF, JAK-STAT and NOD like receptor signaling pathways. Several DEGs overlapped across different KEGG pathways indicating a crosstalk between them. The predicted protein-protein interaction among these DEGs suggested, that the recognition of P. multocida LPS or outer membrane components by TLR4 and CD14, results in intracellular signaling via MyD88, IRAKs and/or TRAF6 leading to activation of NFκB and MAPK pathways and associated cytokines.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0179420Titel-ID: cdi_plos_journals_1919492250
- Format
- –
- Schlagworte
- Adapter proteins, Animals, Apoptosis, Arthritis, Bacteriology, Biology and Life Sciences, Biotechnology, Cattle, CD14 antigen, Colony-forming cells, Computer and Information Sciences, Crosstalk, Cytokines, Disease, DNA microarrays, Edema, Female, Gene expression, Gene Expression Profiling - methods, Gene Expression Profiling - veterinary, Gene Expression Regulation, Gene Regulatory Networks, Genetic aspects, Genomes, Genomics, Health aspects, Hemorrhagic septicemia, Host-bacteria relationships, Immune response, Infections, Inflammation, Intracellular signalling, Kinases, Laboratory animals, Liver, Lungs, MAP kinase, MAP Kinase Signaling System, Medicine and Health Sciences, Mice, MyD88 protein, NF-kappa B - genetics, NF-kappa B - metabolism, NF-κB protein, Oligonucleotide Array Sequence Analysis - methods, Oligonucleotide Array Sequence Analysis - veterinary, Pasteurella, Pasteurella Infections - genetics, Pasteurella multocida - pathogenicity, Protein interaction, Protein Interaction Maps, Respiratory diseases, Rodents, Sepsis, Signal transduction, Spleen, TLR4 protein, Toll-like receptors, TRAF6 protein, Tumor necrosis factor-TNF