Details

Autor(en) / Beteiligte

• Rowley, M Jordan
• Rothi, M Hafiz
• Böhmdorfer, Gudrun
• Kuciński, Jan
• Wierzbicki, Andrzej T
• Mittelsten Scheid, Ortrun

Titel

Long-range control of gene expression via RNA-directed DNA methylation

Ist Teil von

• PLoS genetics, 2017-05, Vol.13 (5), p.e1006749-e1006749

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2017

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• RNA-mediated transcriptional silencing, in plants known as RNA-directed DNA methylation (RdDM), is a conserved process where small interfering RNA (siRNA) and long non-coding RNA (lncRNA) help establish repressive chromatin modifications. This process represses transposons and affects the expression of protein-coding genes. We found that in Arabidopsis thaliana AGO4 binding sites are often located distant from genes differentially expressed in ago4. Using Hi-C to compare interactions between genotypes, we show that RdDM-targeted loci have the potential to engage in chromosomal interactions, but these interactions are inhibited in wild-type conditions. In mutants defective in RdDM, the frequency of chromosomal interactions at RdDM targets is increased. This includes increased frequency of interactions between Pol V methylated sites and distal genes that are repressed by RdDM. We propose a model, where RdDM prevents the formation of chromosomal interactions between genes and their distant regulatory elements.