Details

Autor(en) / Beteiligte

• Nadadhur, Aishwarya G
• Emperador Melero, Javier
• Meijer, Marieke
• Schut, Desiree
• Jacobs, Gerbren
• Li, Ka Wan
• Hjorth, J J Johannes
• Meredith, Rhiannon M
• Toonen, Ruud F
• Van Kesteren, Ronald E
• Smit, August B
• Verhage, Matthijs
• Heine, Vivi M
• Blum, Robert

Titel

Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells

Ist Teil von

• PloS one, 2017-06, Vol.12 (6), p.e0178533-e0178533

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory-inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocytes. We show that these cultures have balanced excitatory-inhibitory synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging and mRNA analysis. These simple and robust protocols offer the opportunity for single-cell to multi-level analysis of patient hiPSC-derived cortical excitatory-inhibitory networks; thereby creating advanced tools to study disease mechanisms underlying neurodevelopmental disorders.