PloS one, 2017-05, Vol.12 (5), p.e0178152-e0178152
2017
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- Autor(en) / Beteiligte
- Titel
- Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model
- Ist Teil von
- PloS one, 2017-05, Vol.12 (5), p.e0178152-e0178152
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Benign prostatic hyperplasia (BPH) is the most common urologic disease among elderly men. A well-established in vitro cell model is required to determine the therapeutic mechanism of BPH inflammation. In this study, we attempted to establish an immortalized human prostate stromal cell line by transfecting with HPV-16 E6/E7 and designated as ihPSC. No significant difference was found in fibroblast-like morphology between primary hPSC and ihPSC. The ihPSC possessed a significantly higher cell proliferation rate than primary hPSC. The prostate-specific markers and proteins including cytoskeleton (α-SMA and vimentin) and smooth muscle (calponin), especially the androgen receptor (AR) were also examined in ihPSC, almost identical to the primary hPSC. To create an in vitro model featuring chronic prostatic inflammation, ihPSC was stimulated with IFN-γ+IL-17 and then treated with the high molecular weight hyaluronic acid hylan G-F 20 as an alternative strategy for inhibiting BPH inflammation. Hylan G-F 20 could dose-dependently diminish the inflammation-induced proliferation in ihPSC. The enhanced expressions of inflammatory molecules including IL-1β, IL-6, IL-8, cyclooxygenase 2 (COX2), inducible nitrogen oxide synthase (iNOS), and Toll-like receptor 4 (TLR4) were all abolished by hylan G-F 20. For inflammatory signaling, hylan G-F 20 can also diminish the IFN-γ+IL-17-increased expression of iNOS and p65 in ihPSC. These findings suggest that ihPSC could provide a mechanism-based platform for investigating prostate inflammation. The hylan G-F 20 showed strong anti-inflammatory effects by decreasing inflammatory cytokines and signalings in the ihPSC, indicating its therapeutic potentials in BPH treatment in the future.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0178152Titel-ID: cdi_plos_journals_1903910819
- Format
- –
- Schlagworte
- Acids, Activation, Age, Anaphylatoxin C5a, Androgen receptors, Animals, Antibiotics, Anticancer properties, Antitumor activity, Arthritis, Bcl protein, Bcl-2 protein, Benign, Biocompatibility, Biology and Life Sciences, Biomedical engineering, Biomedical materials, Biotechnology, Blood vessels, Carbon dioxide, Care and treatment, Cartilage, Cartilage diseases, CD4 antigen, Cell culture, Cell growth, Cell Line, Transformed, Cell lines, Cell proliferation, Chemotherapy, Clinical medicine, Complement component C5a, Complications and side effects, Cultivation, Cytokines, Dentistry, Development and progression, Diagnosis, Differentiation, Dosage and administration, Drug therapy, Engineering, Epithelium, Fragmentation, Genetic aspects, Growth factors, Growth hormones, Health risks, HeLa Cells, Histology, Hospitals, Human papillomavirus, Humans, Hyaluronic acid, Hyaluronic Acid - pharmacology, Hyperplasia, Hypersensitivity, Immune system, Immunology, Incidence, Infiltration, Inflammation, Inflammation Mediators - metabolism, Inhibition, Interferon, Interleukin 1, Interleukin 6, Lymphocytes B, Male, Medical research, Medicine, Medicine and Health Sciences, Metastases, Mice, Mice, Inbred NOD, Mice, SCID, Models, Biological, Molecular weight, Older people, Osteoarthritis, Physiological aspects, Positive feedback, Preservation, Prostate - metabolism, Prostate cancer, Prostatic hyperplasia, Prostatitis - prevention & control, Proteins, Risk, Risk reduction, Stem cells, Stromal Cells - metabolism, Surgery, Surgical implants, Tissue engineering, Toll-like receptors, Urology, Weight reduction