PloS one, 2017-04, Vol.12 (4), p.e0174015
2017
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- Autor(en) / Beteiligte
- Titel
- Molecular actions of two synthetic brassinosteroids, iso-carbaBL and 6-deoxoBL, which cause altered physiological activities between Arabidopsis and rice
- Ist Teil von
- PloS one, 2017-04, Vol.12 (4), p.e0174015
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Brassinosteroid (BR) is an important plant hormone that is perceived by the BRASSINOSTEROID INSENSITIVE 1 (BRI1) receptor. BRI1 is conserved among dicot and monocot species; however, the molecular mechanism underlying BR perception in monocots is not fully understood. We synthesised two BRs, iso-carbabrassinolide (iso-carbaBL) and 6-deoxoBL, which have different BR activities in Arabidopsis thaliana (Arabidopsis) and rice. Our bioassay indicated that iso-carbaBL has relatively strong BR activity in Arabidopsis, but is inactive in rice and competitively inhibits BR activity. The bioactivity of 6-deoxoBL was similar to that of BL in Arabidopsis, but was much lower in rice. Binding experiments using recombinant Arabidopsis and rice BRI1 protein fragments suggested that iso-carbaBL and 6-deoxoBL bind to both receptors. These results showed that iso-carbaBL and 6-deoxoBL act as an antagonist and agonist, respectively, of BRs in rice. A docking simulation analysis suggested that iso-carbaBL fits deeper in the binding pocket to block the binding of active BR to rice BRI1. The simulated binding energy of 6-deoxoBL with rice BRI1 is much lower than that with Arabidopsis BRI1. The possible structural characteristics of rice BRI1 were determined based on the difference in the BR activities of iso-carbaBL and 6-deoxoBL in Arabidopsis and rice.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0174015Titel-ID: cdi_plos_journals_1883868253
- Format
- –
- Schlagworte
- Abscisic acid, Activation, Activation analysis, Agar, Agronomy, Alignment, Alternating current, Amber, Amino acids, Arabidopsis - drug effects, Arabidopsis - genetics, Arabidopsis - physiology, Arabidopsis Proteins - chemistry, Arabidopsis Proteins - metabolism, Arabidopsis thaliana, Assaying, Binding energy, Binding Sites, Bioassays, Biological effects, Biology, Biology and Life Sciences, Biotechnology, Biotin, Brassinolide, Brassinosteroids, Brassinosteroids - chemistry, Brassinosteroids - metabolism, Brassinosteroids - pharmacology, BRI1 protein, Chromatin, Conformational analysis, Crystal structure, Crystals, Cytochrome, Cytochrome P450, Deficient mutant, Dynamic structural analysis, Elongation, Embryogenesis, Embryonic growth stage, Etiolation, Feedback, Free energy, Gene expression, Gene Expression - drug effects, Genes, Plant - drug effects, Homeostasis, Homology, Hormones, Kinases, Life sciences, Mathematical analysis, Matrices (mathematics), Molecular Docking Simulation, Nucleic acids, Nucleotide sequence, Oryza - drug effects, Oryza - genetics, Oryza - physiology, Physical Sciences, Physiological aspects, Plant growth, Plant Growth Regulators - chemistry, Plant Growth Regulators - metabolism, Plant Growth Regulators - pharmacology, Plant hormones, Properties, Protein Kinases - chemistry, Protein Kinases - metabolism, Protein structure, Protein-Serine-Threonine Kinases - chemistry, Protein-Serine-Threonine Kinases - metabolism, Protein-serine/threonine kinase, Proteins, Research and Analysis Methods, Residues, Rice, Simulation, Species Specificity, Steroids (Organic compounds), Sugar, Superhelix, Transcription