PloS one, 2017-03, Vol.12 (3), p.e0173438-e0173438
2017
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Details
- Autor(en) / Beteiligte
- Titel
- RNA-sequence analysis of gene expression from honeybees (Apis mellifera) infected with Nosema ceranae
- Ist Teil von
- PloS one, 2017-03, Vol.12 (3), p.e0173438-e0173438
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Honeybees (Apis mellifera) are constantly subjected to many biotic stressors including parasites. This study examined honeybees infected with Nosema ceranae (N. ceranae). N. ceranae infection increases the bees energy requirements and may contribute to their decreased survival. RNA-seq was used to investigate gene expression at days 5, 10 and 15 Post Infection (P.I) with N. ceranae. The expression levels of genes, isoforms, alternative transcription start sites (TSS) and differential promoter usage revealed a complex pattern of transcriptional and post-transcriptional gene regulation suggesting that bees use a range of tactics to cope with the stress of N. ceranae infection. N. ceranae infection may cause reduced immune function in the bees by: (i)disturbing the host amino acids metabolism (ii) down-regulating expression of antimicrobial peptides (iii) down-regulation of cuticle coatings and (iv) down-regulation of odorant binding proteins.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0173438Titel-ID: cdi_plos_journals_1882248029
- Format
- –
- Schlagworte
- Agricultural ecosystems, Alternative splicing, Amino acids, Animals, Antimicrobial peptides, Apis mellifera, Bacteria, Bacterial infections, Bees, Bees - genetics, Bees - microbiology, Biochemistry, Biology and Life Sciences, Defense mechanisms, Defensive behavior, Digestive tract, Diseases, Energy Metabolism - genetics, European honeybee, Fungi, Gene expression, Gene Expression Profiling - methods, Genes, Insect - genetics, Genetic aspects, Genetics, Genomes, Genomics, Hemolymph, Honeybee, Host-Pathogen Interactions, Immune response, Immune response (humoral), Immunology, Immunosuppression, Infections, Insect Proteins - genetics, Insects, Life Sciences, Longevity, Medicine and Health Sciences, Microorganisms, Molecular biology, Muscles, Nervous system, Nosema - physiology, Nosema ceranae, Nucleotide sequence, Nutrition, Overwintering, Parasites, Pathology, Peptides, Pollen, Principal Component Analysis, Promoter Regions, Genetic - genetics, Protein Isoforms - genetics, Proteins, Reverse Transcriptase Polymerase Chain Reaction, Ribonucleic acid, Risk factors, RNA, RNA sequencing, Sequence Analysis, RNA - methods, Side effects, Signal transduction, Signal Transduction - genetics, Spectrophotometry, Spores, Fungal - physiology, Studies, Sucrose, Time Factors, Transcription Initiation Site