Details
- Autor(en) / Beteiligte
- Titel
- Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy
- Ist Teil von
- PloS one, 2017-01, Vol.12 (1), p.e0170442
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cancer cell toxicity to therapeutic H2O2 varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2 concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H2O2 doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0170442Titel-ID: cdi_plos_journals_1860295651
- Format
- –
- Schlagworte
- Apoptosis, Aquaporin 3, Aquaporin 3 - genetics, Aquaporin 3 - metabolism, Aquaporins, Ascorbic acid, Ascorbic Acid - pharmacology, Bioengineering, Biology and Life Sciences, Cancer, Cancer therapies, Cell Line, Tumor, Cell Survival, Cytotoxicity, Deoxyribonucleic acid, DNA, DNA damage, Enzymes, Free radicals, Gene Silencing, Humans, Hydrogen peroxide, Hydrogen Peroxide - pharmacology, Immunohistochemistry, Medicine and Health Sciences, Metabolism, Neoplasms - pathology, Pancreatic cancer, Permeability, Pharmacology, Physical sciences, Physiology, Plasma, Research and Analysis Methods, Therapy, Toxicity