PloS one, 2016-11, Vol.11 (11), p.e0165776-e0165776
2016
Details
- Autor(en) / Beteiligte
- Titel
- MicroRNA-93 Promotes Epithelial-Mesenchymal Transition of Endometrial Carcinoma Cells
- Ist Teil von
- PloS one, 2016-11, Vol.11 (11), p.e0165776-e0165776
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- MicroRNA-93, derived from a paralog (miR-106b-25) of the miR-17-92 cluster, is involved in the tumorigenesis and progression of many cancers such as breast, colorectal, hepatocellular, lung, ovarian, and pancreatic cancer. However, the role of miR-93 in endometrial carcinoma and the potential molecular mechanisms involved remain unknown. Our results showed that miR-93 was overexpressed in endometrial carcinoma tissues than normal endometrial tissues. The endometrial carcinoma cell lines HEC-1B and Ishikawa were transfected with miR-93-5P, after which cell migration and invasion ability and the expression of relevant molecules were detected. MiR-93 overexpression promoted cell migration and invasion, and downregulated E-cadherin expression while increasing N-cadherin expression. Dual-luciferase reporter assay showed that miR-93 may directly bind to the 3' untranslated region of forkhead box A1 (FOXA1); furthermore, miR-93 overexpression downregulated FOXA1 expression while miR-93 inhibitor transfection upregulated FOXA1 expression at both mRNA and protein level. In addition, transfection with the most effective FOXA1 small interfering RNA promoted both endometrial cancer cell migration and invasion, and downregulated E-cadherin expression while upregulating N-cadherin expression. Therefore, we suggest that miR-93 may promote the process of epithelial-mesenchymal transition in endometrial carcinoma cells by targeting FOXA1.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0165776Titel-ID: cdi_plos_journals_1837599042
- Format
- –
- Schlagworte
- 3' Untranslated Regions, Antigens, CD - genetics, Antigens, CD - metabolism, B cells, Binding Sites, Biology and Life Sciences, Breast cancer, Cadherins - genetics, Cadherins - metabolism, Cancer, Cancer metastasis, Carcinoma, Cell adhesion & migration, Cell growth, Cell Line, Tumor, Cell migration, Cell Movement, Cell Transformation, Neoplastic - genetics, Cell Transformation, Neoplastic - metabolism, Cell Transformation, Neoplastic - pathology, E-cadherin, Endometrial cancer, Endometrial Neoplasms - genetics, Endometrial Neoplasms - metabolism, Endometrial Neoplasms - pathology, Endometrium, Endometrium - metabolism, Endometrium - pathology, Epithelial-Mesenchymal Transition - genetics, Female, Forkhead protein, Gene expression, Gene Expression Regulation, Neoplastic, Genes, Reporter, Growth factors, Gynecology, HEK293 Cells, Hepatocyte Nuclear Factor 3-alpha - antagonists & inhibitors, Hepatocyte Nuclear Factor 3-alpha - genetics, Hepatocyte Nuclear Factor 3-alpha - metabolism, Humans, Luciferase, Luciferases - genetics, Luciferases - metabolism, Lung cancer, Medicine and Health Sciences, Membranes, Mesenchyme, Metastasis, MicroRNA, MicroRNAs, MicroRNAs - genetics, MicroRNAs - metabolism, miRNA, Molecular modelling, mRNA, N-Cadherin, Ovarian cancer, Pancreatic cancer, Prostate cancer, Research and Analysis Methods, Ribonucleic acid, RNA, RNA, Small Interfering - genetics, RNA, Small Interfering - metabolism, Signal Transduction, siRNA, Stem cells, Tissues, Transfection, Tumor cell lines, Tumorigenesis, Uterine cancer