PloS one, 2016-10, Vol.11 (10), p.e0164751-e0164751
2016
Details
- Autor(en) / Beteiligte
- Titel
- Distinctive Regulatory T Cells and Altered Cytokine Profile Locally in the Airways of Young Smokers with Normal Lung Function
- Ist Teil von
- PloS one, 2016-10, Vol.11 (10), p.e0164751-e0164751
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Smoking influences the immune system in different ways and, hypothetically, effects on pulmonary effector and regulatory T cells emerge as potentially detrimental. Therefore, we characterized the frequencies and characteristics of CD4+ and CD8+ T cell subsets in the blood and lungs of young tobacco smokers. Bronchoalveolar lavage (BAL) and peripheral blood were obtained from healthy moderate smokers (n = 18; 2-24 pack-years) and never-smokers (n = 15), all with normal lung function. Cells were stimulated ex vivo and key intracellular cytokines (IFNγ, IL-17, IL-10 and TNFα) and transcription factors (Foxp3, T-bet and Helios) were analyzed using flow cytometry. Our results indicate that smoking is associated with a decline in lung IL-17+ CD4+ T cells, increased IFNγ+ CD8+ T cells and these alterations relate to the history of daily cigarette consumption. There is an increased fraction of Foxp3+ regulatory T cells being Helios- in the lungs of smokers. Cytokine production is mainly confined to the Helios- T cells, both in regulatory and effector subsets. Moreover, we detected a decline of Helios+Foxp3- postulated regulatory CD8+ T cells in smokers. These alterations in the immune system are likely to increase risk for infection and may have implications for autoimmune processes initiated in the lungs among tobacco smokers.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0164751Titel-ID: cdi_plos_journals_1834382394
- Format
- –
- Schlagworte
- Adult, Alterations, Alveoli, Analysis, Arthritis, Biology and Life Sciences, Biomarkers, Blood, Bronchoalveolar lavage, Bronchoalveolar Lavage Fluid, Bronchus, CD4 antigen, CD8 antigen, Chronic obstructive pulmonary disease, Cytokines, Cytokines - biosynthesis, Cytometry, Disease, Effector cells, Female, Flow cytometry, Foxp3 protein, Gene expression, Health risks, Hospitals, Humans, Immune system, Immunology, Immunophenotyping, Immunoregulation, Infections, Inflammation, Interleukin 10, Interleukin 17, Leukocytes, Mononuclear - immunology, Leukocytes, Mononuclear - metabolism, Lung - metabolism, Lungs, Lymphocytes, Lymphocytes T, Male, Medicin och hälsovetenskap, Medicine, Medicine and Health Sciences, Middle Aged, Pathogenesis, Peripheral blood, Phenotype, Pneumoviridae, Pulmonary Disease, Chronic Obstructive - immunology, Pulmonary Disease, Chronic Obstructive - metabolism, Research and Analysis Methods, Respiratory function, Respiratory Function Tests, Respiratory System - metabolism, Rheumatology, Smokers, Smoking, Smoking and youth, T cell receptors, T cells, T-Lymphocyte Subsets - immunology, T-Lymphocyte Subsets - metabolism, T-Lymphocytes, Regulatory - immunology, T-Lymphocytes, Regulatory - metabolism, Tobacco, Transcription factors, Transcription Factors - metabolism, Tumor necrosis factor, Tumor necrosis factor-α, Young Adult, Youth smoking, γ-Interferon