Details

Autor(en) / Beteiligte

• Patel, Pranav
• Abd El Wahed, Ahmed
• Faye, Oumar
• Prüger, Pauline
• Kaiser, Marco
• Thaloengsok, Sasikanya
• Ubol, Sukathida
• Sakuntabhai, Anavaj
• Leparc-Goffart, Isabelle
• Hufert, Frank T
• Sall, Amadou A
• Weidmann, Manfred
• Niedrig, Matthias
• Williams, Maya

Titel

A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus

Ist Teil von

• PLoS neglected tropical diseases, 2016-09, Vol.10 (9), p.e0004953-e0004953

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2016

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis. In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%. The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need.