PloS one, 2016-08, Vol.11 (8), p.e0161826-e0161826
2016
Details
- Autor(en) / Beteiligte
- Titel
- Effect of IVIG Formulation on IgG Binding to Self- and Exo- Antigens In Vitro and In Vivo
- Ist Teil von
- PloS one, 2016-08, Vol.11 (8), p.e0161826-e0161826
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- In relation to the recent trials of Intravenous Immunoglobulin (IVIG) in Alzheimer's Disease (AD) it was demonstrated that different IgG preparations contain varying amounts of natural anti-amyloid β (Aβ) antibodies as measured by ELISA. We therefore investigated the relevance of ELISA data for measuring low-affinity antibodies, such as anti-Aβ. We analysed the binding of different commercial Immunoglobulin G (IgG) preparations to Aβ, actin and tetanus toxoid in different binding assays to further investigate the possible cause for observed differences in binding to Aβ and actin between different IgG preparations. We show that the differences of commercial IgG preparations in binding to Aβ and actin in ELISA assays are artefactual and only evident in in vitro binding assays. In functional assays and in vivo animal studies the different IVIG preparations exhibited very similar potency. ELISA data alone are not appropriate to analyse and rank the binding capacity of low-affinity antibodies to Aβ or other endogenous self-antigens contained in IgG preparations. Additional analytical methods should be adopted to complement ELISA data.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0161826Titel-ID: cdi_plos_journals_1814156105
- Format
- –
- Schlagworte
- Actin, Actins - immunology, Actins - metabolism, Affinity, Alzheimer Disease - drug therapy, Alzheimer Disease - immunology, Alzheimer Disease - metabolism, Alzheimer's disease, Alzheimers disease, Amyloid, Amyloid beta-Peptides - immunology, Amyloid beta-Peptides - metabolism, Analytical methods, Animals, Antibodies, Antibody Affinity - drug effects, Antibody Affinity - immunology, Antigens, Antigens - immunology, Antigens - metabolism, Assaying, Autoantigens, Autoantigens - immunology, Autoantigens - metabolism, Binding, Biology and Life Sciences, Blood & organ donations, Chemical properties, Complications and side effects, Dosage and administration, Drug therapy, Enzyme-Linked Immunosorbent Assay, Female, Genetic aspects, Guillain-Barre syndrome, Herpesvirus 3, Human - immunology, Herpesvirus 3, Human - metabolism, Humans, Immunoglobulin G, Immunoglobulin G - immunology, Immunoglobulin G - metabolism, Immunoglobulins, Immunoglobulins, Intravenous - immunology, Immunoglobulins, Intravenous - pharmacology, In vivo methods and tests, Intravenous administration, Intravenous immunoglobulins, Medicine and Health Sciences, Neurodegenerative diseases, Peptide Fragments - immunology, Peptide Fragments - metabolism, Peptides, Physical Sciences, Physiological aspects, Protein Binding - drug effects, Protein Binding - immunology, Rats, Research and Analysis Methods, Tetanus, Tetanus Toxoid - immunology, Tetanus Toxoid - metabolism, Vaccines