Details

Autor(en) / Beteiligte

• Resch, Yvonne
• Blatt, Katharina
• Malkus, Ursula
• Fercher, Christian
• Swoboda, Ines
• Focke-Tejkl, Margit
• Chen, Kuan-Wei
• Seiberler, Susanne
• Mittermann, Irene
• Lupinek, Christian
• Rodriguez-Dominguez, Azahara
• Zieglmayer, Petra
• Zieglmayer, René
• Keller, Walter
• Krzyzanek, Vladislav
• Valent, Peter
• Valenta, Rudolf
• Vrtala, Susanne
• Fritz, Jörg Hermann

Titel

Molecular, Structural and Immunological Characterization of Der p 18, a Chitinase-Like House Dust Mite Allergen

Ist Teil von

• PloS one, 2016-08, Vol.11 (8), p.e0160641-e0160641

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• The house dust mite (HDM) allergen Der p 18 belongs to the glycoside hydrolase family 18 chitinases. The relevance of Der p 18 for house dust mite allergic patients has only been partly investigated. To perform a detailed characterization of Der p 18 on a molecular, structural and immunological level. Der p 18 was expressed in E. coli, purified to homogeneity, tested for chitin-binding activity and its secondary structure was analyzed by circular dichroism. Der p 18-specific IgG antibodies were produced in rabbits to localize the allergen in mites using immunogold electron microscopy and to search for cross-reactive allergens in other allergen sources (i.e. mites, crustacea, mollusca and insects). IgE reactivity of rDer p 18 was tested with sera from clinically well characterized HDM-allergic patients (n = 98) and its allergenic activity was analyzed in basophil activation experiments. Recombinant Der p 18 was expressed and purified as a folded, biologically active protein. It shows weak chitin-binding activity and partial cross-reactivity with Der f 18 from D. farinae but not with proteins from the other tested allergen sources. The allergen was mainly localized in the peritrophic matrix of the HDM gut and to a lower extent in fecal pellets. Der p 18 reacted with IgE from 10% of mite allergic patients from Austria and showed allergenic activity when tested for basophil activation in Der p 18-sensitized patients. Der p 18 is a rather genus-specific minor allergen with weak chitin-binding activity but exhibits allergenic activity and therefore should be included in diagnostic test panels for HDM allergy.