PloS one, 2016-08, Vol.11 (8), p.e0160887-e0160887
2016
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- Autor(en) / Beteiligte
- Titel
- miR-17-3p Exacerbates Oxidative Damage in Human Retinal Pigment Epithelial Cells
- Ist Teil von
- PloS one, 2016-08, Vol.11 (8), p.e0160887-e0160887
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Oxidative stress has been shown to contribute to the development of age-related macular degeneration (AMD). MicroRNAs (miRNA) are small non-coding RNA molecules that function in RNA silencing and post-transcriptional regulation of gene expression. We showed miR-17-3p to be elevated in macular RPE cells from AMD patients and in ARPE-19 cells under oxidative stress. Transfection of miR-17-3p mimic in ARPE-19 induced cell death and exacerbated oxidative lethality that was alleviated by miR-17-3p inhibitor. The expression of antioxidant enzymes manganese superoxide dismutase (MnSOD) and thioredoxin reductase-2 (TrxR2) were suppressed by miR-17-3p mimic and reversed by miR-17-3p inhibitor. These results suggest miR-17-3p aggravates oxidative damage-induced cell death in human RPE cells, while miR-17-3p inhibitor acts as a potential protector against oxidative stress by regulating the expression of antioxidant enzymes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0160887Titel-ID: cdi_plos_journals_1812536362
- Format
- –
- Schlagworte
- Age, Aged, Aged, 80 and over, Alzheimer's disease, Angiogenesis, Antioxidants, Antioxidants - metabolism, Apoptosis, Biology and Life Sciences, Blindness, Care and treatment, Cell death, Cell growth, Cell Survival - genetics, Complications and side effects, Enzymes, Gene expression, Gene Expression Regulation, Enzymologic - genetics, Gene regulation, Gene silencing, Humans, Inflammation, Influence, Inhibitors, Laboratories, Lethality, Macular degeneration, Macular Degeneration - genetics, Macular Degeneration - pathology, Manganese, Medical schools, Medicine and Health Sciences, MicroRNA, MicroRNAs, MicroRNAs - genetics, miRNA, Non-coding RNA, Oxidative stress, Oxidative Stress - genetics, Pathogenesis, Photoreceptors, Post-transcription, Reductase, Research and Analysis Methods, Retina, Retinal pigment epithelium, Retinal Pigment Epithelium - enzymology, Retinal Pigment Epithelium - metabolism, Ribonucleic acid, Risk factors, RNA, RNA-mediated interference, Rodents, Superoxide dismutase, Superoxide Dismutase - metabolism, Thioredoxin, Thioredoxin Reductase 2 - metabolism, Transfection