PloS one, 2016-08, Vol.11 (8), p.e0160341-e0160341
2016
Details
- Autor(en) / Beteiligte
- Titel
- HIV-1-Specific Antibody Response and Function after DNA Prime and Recombinant Adenovirus 5 Boost HIV Vaccine in HIV-Infected Subjects
- Ist Teil von
- PloS one, 2016-08, Vol.11 (8), p.e0160341-e0160341
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- Little is known about the humoral immune response against DNA prime-recombinant adenovirus 5 (rAd5) boost HIV vaccine among HIV-infected patients on long-term suppressive antiretroviral therapy (ART). Previous studies emphasized cellular immune responses; however, current research suggests both cellular and humoral responses are likely required for a successful therapeutic vaccine. Thus, we aimed to understand antibody response and function induced by vaccination of ART-treated HIV-1-infected patients with immune recovery. All subjects participated in EraMune 02, an open-label randomized clinical trial of ART intensification followed by a six plasmid DNA prime (envA, envB, envC, gagB, polB, nefB) and rAd5 boost HIV vaccine with matching inserts. Antibody binding levels were determined with a recently developed microarray approach. We also analyzed neutralization efficiency and antibody-dependent cellular cytotoxicity (ADCC). We found that the DNA prime-rAd5 boost vaccine induced a significant cross-clade HIV-specific antibody response, which correlated with antibody neutralization efficiency. However, despite the increase in antibody binding levels, the vaccine did not significantly stimulate neutralization or ADCC responses. This finding was also reflected by a lack of change in total CD4+ cell associated HIV DNA in those who received the vaccine. Our results have important implications for further therapeutic vaccine design and administration, especially in HIV-1 infected patients, as boosting of preexisting antibody responses are unlikely to lead to clearance of latent proviruses in the HIV reservoir.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0160341Titel-ID: cdi_plos_journals_1812534555
- Format
- –
- Schlagworte
- Acquired immune deficiency syndrome, Adenoviridae, Adenoviridae - genetics, Adenoviridae - immunology, Adenovirus, Adenoviruses, AIDS, AIDS vaccines, AIDS Vaccines - administration & dosage, AIDS Vaccines - immunology, Antibodies, Neutralizing - blood, Antibodies, Neutralizing - immunology, Antibodies, Viral - blood, Antibodies, Viral - immunology, Antibody response, Antiretroviral agents, Antiretroviral therapy, Antiviral agents, Binding, Biology and Life Sciences, Care and treatment, CD4 antigen, CD4-Positive T-Lymphocytes, Clinical trials, Cytotoxicity, Deoxyribonucleic acid, DNA, DNA microarrays, DNA vaccines, Female, Health aspects, Highly active antiretroviral therapy, HIV, HIV (Viruses), HIV Antibodies - blood, HIV Antibodies - immunology, HIV infection, HIV infections, HIV Infections - drug therapy, HIV Infections - immunology, HIV Infections - virology, HIV patients, HIV-1 - genetics, HIV-1 - immunology, Hospitals, Human immunodeficiency virus, Human immunodeficiency virus 1, Human Immunodeficiency Virus Proteins - immunology, Humans, Immune clearance, Immune response, Immune response (cell-mediated), Immune response (humoral), Immune system, Immunization, Secondary - methods, Immunoglobulins, Immunology, Infections, Infectious diseases, Inserts, Lentivirus, Male, Medical research, Medicine, Medicine and Health Sciences, Medicine, Experimental, Middle Aged, Neutralization, Patients, Plasmids, Proviruses, Research and Analysis Methods, Retroviridae, Toxicity, Vaccination, Vaccines, Vaccines, DNA - administration & dosage, Vaccines, DNA - immunology, Virology, Viruses