PloS one, 2016-06, Vol.11 (6), p.e0156228-e0156228
2016
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Details
- Autor(en) / Beteiligte
- Titel
- Efficient Virus-Induced Gene Silencing in Solanum rostratum
- Ist Teil von
- PloS one, 2016-06, Vol.11 (6), p.e0156228-e0156228
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Solanum rostratum is a "super weed" that grows fast, is widespread, and produces the toxin solanine, which is harmful to both humans and other animals. To our knowledge, no study has focused on its molecular biology owing to the lack of available transgenic methods and sequence information for S. rostratum. Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function in plants; therefore, in the present study, we aimed to establish tobacco rattle virus (TRV)-derived VIGS in S. rostratum. The genes for phytoene desaturase (PDS) and Chlorophyll H subunit (ChlH) of magnesium protoporphyrin chelatase were cloned from S. rostratum and used as reporters of gene silencing. It was shown that high-efficiency VIGS can be achieved in the leaves, flowers, and fruit of S. rostratum. Moreover, based on our comparison of three different types of infection methods, true leaf infection was found to be more efficient than cotyledon and sprout infiltration in long-term VIGS in multiple plant organs. In conclusion, the VIGS technology and tomato genomic sequences can be used in the future to study gene function in S. rostratum.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0156228Titel-ID: cdi_plos_journals_1793753312
- Format
- –
- Schlagworte
- Biology and Life Sciences, Biotechnology, Chlorophyll, Desaturase, Engineering, Flowers, Flowers & plants, Flowers - genetics, Flowers - metabolism, Food science, Fruit - genetics, Fruit - metabolism, Gene Expression Regulation, Plant - genetics, Gene sequencing, Gene silencing, Gene Silencing - physiology, Genes, Genetic aspects, Genomes, Infections, Infiltration, Laboratories, Leaves, Lycopersicon esculentum, Magnesium, Methods, Molecular biology, Organs, Oxidoreductases - genetics, Oxidoreductases - metabolism, Physiological aspects, Plant Leaves - genetics, Plant Leaves - metabolism, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Viruses - genetics, Plant Viruses - physiology, Plants (botany), Protoporphyrin, Seeds, Solanum, Solanum - genetics, Solanum rostratum, Tobacco, Tobacco rattle virus, Tobravirus, Tomatoes, Viruses