PloS one, 2016-05, Vol.11 (5), p.e0156727-e0156727
2016
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- Autor(en) / Beteiligte
- Titel
- Novel Detection of Coxiella spp., Theileria luwenshuni, and T. ovis Endosymbionts in Deer Keds (Lipoptena fortisetosa)
- Ist Teil von
- PloS one, 2016-05, Vol.11 (5), p.e0156727-e0156727
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We describe for the first time the detection of Coxiella-like bacteria (CLB), Theileria luwenshuni, and T. ovis endosymbionts in blood-sucking deer keds. Eight deer keds attached to a Korean water deer were identified as Lipoptena fortisetosa (Diptera: Hippoboscidae) by morphological and genetic analyses. Among the endosymbionts assessed, CLB, Theileria luwenshuni, and T. ovis were identified in L. fortisetosa by PCR and nucleotide sequencing. Based on phylogeny, CLB 16S rRNA sequences were classified into clade B, sharing 99.4% identity with CLB from Haemaphysalis longicornis in South Korea. Although the virulence of CLB to vertebrates is still controversial, several studies have reported clinical symptoms in birds due to CLB infections. The 18S rRNA sequences of T. luwenshuni and T. ovis in this study were 98.8-100% identical to those in GenBank, and all of the obtained sequences of T. ovis and T. luwenshuni in this study were 100% identical to each other, respectively. Although further studies are required to positively confirm L. fortisetosa as a biological vector of these pathogens, strong genetic relationships among sequences from this and previous studies suggest potential transmission among mammalian hosts by ticks and keds.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0156727Titel-ID: cdi_plos_journals_1792773129
- Format
- –
- Schlagworte
- Anaplasma, Animals, Babesia, Bartonella, Base Sequence, Biology and Life Sciences, Birds, Borrelia, Borrelia burgdorferi, Climate change, Coxiella - classification, Coxiella - isolation & purification, Coxiella burnetii, Deer, Deoxyribonucleic acid, Diptera, Diptera - microbiology, Diptera - parasitology, Disease transmission, DNA, DNA, Bacterial - genetics, DNA, Protozoan - genetics, Ehrlichia, Endosymbionts, Gene sequencing, Genetic analysis, Genetic aspects, Genotypes, Gram-Negative Bacterial Infections - microbiology, Gram-Negative Bacterial Infections - transmission, Gram-Negative Bacterial Infections - veterinary, Haemaphysalis longicornis, Health aspects, Hepatozoon, Hippoboscidae, Identification and classification, Infections, Ixodidae, Lipoptena, Lipoptena cervi, Maximum likelihood method, Medical research, Medicine and Health Sciences, Ovis, Ovis aries, Pathogens, Phylogenetics, Phylogeny, Polymerase chain reaction, Proteobacteria, Protozoans, Research and Analysis Methods, Rickettsia, RNA, Ribosomal, 16S - genetics, RNA, Ribosomal, 18S - genetics, rRNA 16S, rRNA 18S, Sequence Analysis, DNA, Studies, Symbiosis, Theileria, Theileria - classification, Theileria - genetics, Theileria - isolation & purification, Theileriasis - parasitology, Theileriasis - transmission, Ticks, Vectors (Biology), Vertebrates, Veterinary colleges, Veterinary medicine, Veterinary services, Virulence, Virus-vector relationships