Details

Autor(en) / Beteiligte

• Talhouk, Aline
• Kommoss, Stefan
• Mackenzie, Robertson
• Cheung, Martin
• Leung, Samuel
• Chiu, Derek S
• Kalloger, Steve E
• Huntsman, David G
• Chen, Stephanie
• Intermaggio, Maria
• Gronwald, Jacek
• Chan, Fong C
• Ramus, Susan J
• Steidl, Christian
• Scott, David W
• Anglesio, Michael S
• Haibe-Kains, Benjamin

Titel

Single-Patient Molecular Testing with NanoString nCounter Data Using a Reference-Based Strategy for Batch Effect Correction

Ist Teil von

• PloS one, 2016-04, Vol.11 (4), p.e0153844-e0153844

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• A major weakness in many high-throughput genomic studies is the lack of consideration of a clinical environment where one patient at a time must be evaluated. We examined generalizable and platform-specific sources of variation from NanoString gene expression data on both ovarian cancer and Hodgkin lymphoma patients. A reference-based strategy, applicable to single-patient molecular testing is proposed for batch effect correction. The proposed protocol improved performance in an established Hodgkin lymphoma classifier, reducing batch-to-batch misclassification while retaining accuracy and precision. We suggest this strategy may facilitate development of NanoString and similar molecular assays by accelerating prospective validation and clinical uptake of relevant diagnostics.