PloS one, 2016-03, Vol.11 (3), p.e0151598
2016
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- Autor(en) / Beteiligte
- Titel
- CYP1B1 Enhances Cell Proliferation and Metastasis through Induction of EMT and Activation of Wnt/β-Catenin Signaling via Sp1 Upregulation
- Ist Teil von
- PloS one, 2016-03, Vol.11 (3), p.e0151598
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Cytochrome P450 1B1 (CYP1B1) is a major E2 hydroxylase involved in the metabolism of potential carcinogens. CYP1B1 expression has been reported to be higher in tumors compared to normal tissues, especially in hormone-related cancers including breast, ovary, and prostate tumors. To explore the role of CYP1B1 in cancer progression, we investigated the action of CYP1B1 in cells with increased CYP1B1 via the inducer 7,12-dimethylbenz[α]anthracene (DMBA) or an overexpression vector, in addition to decreased CYP1B1 via the inhibitor tetramethoxystilbene (TMS) or siRNA knockdown. We observed that CYP1B1 promoted cell proliferation, migration, and invasion in MCF-7 and MCF-10A cells. To understand its molecular mechanism, we measured key oncogenic proteins including β-catenin, c-Myc, ZEB2, and matrix metalloproteinases following CYP1B1 modulation. CYP1B1 induced epithelial-mesenchymal transition (EMT) and activated Wnt/β-catenin signaling via upregulation of CTNNB1, ZEB2, SNAI1, and TWIST1. Sp1, a transcription factor involved in cell growth and metastasis, was positively regulated by CYP1B1, and suppression of Sp1 expression by siRNA or DNA binding activity using mithramycin A blocked oncogenic transformation by CYP1B1. Therefore, we suggest that Sp1 acts as a key mediator for CYP1B1 action. Treatment with 4-hydroxyestradiol (4-OHE2), a major metabolite generated by CYP1B1, showed similar effects as CYP1B1 overexpression, indicating that CYP1B1 activity mediated various oncogenic events in cells. In conclusion, our data suggests that CYP1B1 promotes cell proliferation and metastasis by inducing EMT and Wnt/β-catenin signaling via Sp1 induction.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0151598Titel-ID: cdi_plos_journals_1773812854
- Format
- –
- Schlagworte
- 9,10-Dimethyl-1,2-benzanthracene, Adenoviruses, Anthracene, beta Catenin - metabolism, Biology and Life Sciences, Breast cancer, c-Myc protein, Cancer, Carcinogens, Cell adhesion & migration, Cell cycle, Cell growth, Cell Line, Tumor, Cell migration, Cell proliferation, Cell Proliferation - drug effects, Cytochrome, Cytochrome P-450 CYP1B1 - antagonists & inhibitors, Cytochrome P-450 CYP1B1 - biosynthesis, Cytochrome P-450 CYP1B1 - genetics, Cytochrome P-450 CYP1B1 - physiology, Cytochrome P450, Deoxyribonucleic acid, DNA, Epithelial-Mesenchymal Transition, Estrogens, Catechol - metabolism, Gene expression, Genes, Humans, Hydroxylase, Matrix metalloproteinases, Medicine and Health Sciences, Mesenchyme, Metabolism, Metastases, Metastasis, MicroRNAs, Myc protein, Neoplasm Metastasis, Penicillin, Pharmacy, Prostate, Prostate cancer, Proteins, Research and Analysis Methods, Signal Transduction, Signaling, siRNA, Snail protein, Sp1 protein, Sp1 Transcription Factor - metabolism, Tissues, Transcription factors, Transformation, Tumors, Up-Regulation, Wnt protein, Wnt Proteins - metabolism, β-Catenin