PloS one, 2016-02, Vol.11 (2), p.e0148684-e0148684
2016
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Details
- Autor(en) / Beteiligte
- Titel
- Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence
- Ist Teil von
- PloS one, 2016-02, Vol.11 (2), p.e0148684-e0148684
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Direct measurement of insulin is critical for basic and clinical studies of insulin secretion. However, current methods are expensive and time-consuming. We developed an insulin assay based on homogenous time-resolved fluorescence that is significantly more rapid and cost-effective than current commonly used approaches. This assay was applied effectively to an insulin secreting cell line, INS-1E cells, as well as pancreatic islets, allowing us to validate the assay by elucidating mechanisms by which dopamine regulates insulin release. We found that dopamine functioned as a significant negative modulator of glucose-stimulated insulin secretion. Further, we showed that bromocriptine, a known dopamine D2/D3 receptor agonist and newly approved drug used for treatment of type II diabetes mellitus, also decreased glucose-stimulated insulin secretion in islets to levels comparable to those caused by dopamine treatment.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0148684Titel-ID: cdi_plos_journals_1762831801
- Format
- –
- Schlagworte
- Animals, Assaying, Bioassays, Biological assays, Biology and Life Sciences, Bromocriptine, Bromocriptine - chemistry, Bromocriptine - pharmacology, Cell Line, Cell lines, Diabetes, Diabetes mellitus, Dopamine, Dopamine D2 receptors, Dopamine D3 receptors, Endocrinology, Fluorescence, Glucose, Insulin, Insulin - analysis, Insulin - metabolism, Insulin resistance, Insulin Secretion, Insulin-Secreting Cells - cytology, Insulin-Secreting Cells - metabolism, Kinases, Measurement, Medicine and Health Sciences, Metabolism, Mice, Obesity, Pancreas, Physical Sciences, Physiological aspects, Psychiatry, Rats, Receptors, Dopamine D2 - agonists, Receptors, Dopamine D2 - metabolism, Receptors, Dopamine D3 - agonists, Receptors, Dopamine D3 - metabolism, Research and Analysis Methods, Rodents, Secretion, Surgeons