PloS one, 2015-09, Vol.10 (9), p.e0138540
2015
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- Autor(en) / Beteiligte
- Titel
- Genome-Wide Identification of the Invertase Gene Family in Populus
- Ist Teil von
- PloS one, 2015-09, Vol.10 (9), p.e0138540
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Invertase plays a crucial role in carbohydrate partitioning and plant development as it catalyses the irreversible hydrolysis of sucrose into glucose and fructose. The invertase family in plants is composed of two sub-families: acid invertases, which are targeted to the cell wall and vacuole; and neutral/alkaline invertases, which function in the cytosol. In this study, 5 cell wall invertase genes (PtCWINV1-5), 3 vacuolar invertase genes (PtVINV1-3) and 16 neutral/alkaline invertase genes (PtNINV1-16) were identified in the Populus genome and found to be distributed on 14 chromosomes. A comprehensive analysis of poplar invertase genes was performed, including structures, chromosome location, phylogeny, evolutionary pattern and expression profiles. Phylogenetic analysis indicated that the two sub-families were both divided into two clades. Segmental duplication is contributed to neutral/alkaline sub-family expansion. Furthermore, the Populus invertase genes displayed differential expression in roots, stems, leaves, leaf buds and in response to salt/cold stress and pathogen infection. In addition, the analysis of enzyme activity and sugar content revealed that invertase genes play key roles in the sucrose metabolism of various tissues and organs in poplar. This work lays the foundation for future functional analysis of the invertase genes in Populus and other woody perennials.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0138540Titel-ID: cdi_plos_journals_1719292518
- Format
- –
- Schlagworte
- Amino Acid Sequence, Amino acids, Ascomycota - physiology, beta-Fructofuranosidase - classification, beta-Fructofuranosidase - genetics, beta-Fructofuranosidase - metabolism, Biological evolution, Biotechnology, Carbohydrates, Cell walls, Chromosome Mapping, Chromosomes, Chromosomes, Plant - genetics, Cladistic analysis, Cold Temperature, Cytosol, Education, Engineering, Enzymatic activity, Enzyme activity, Enzymes, Forestry, Fructose, Functional analysis, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Enzymologic - drug effects, Gene Expression Regulation, Plant - drug effects, Genes, Genome, Plant - genetics, Genomes, Genomics, Glucose, Host-Pathogen Interactions, Hydrolysis, Identification, Infections, Invertase, Isoenzymes - genetics, Laboratories, Leaves, Metabolism, Molecular biology, Molecular Sequence Data, Multigene Family, Organs, Pathogens, Phylogenetics, Phylogeny, Physiology, Plant biology, Plant Leaves - enzymology, Plant Leaves - genetics, Plant Leaves - microbiology, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Roots - enzymology, Plant Roots - genetics, Plant Roots - microbiology, Plant tissues, Poplar, Populus - enzymology, Populus - genetics, Populus - microbiology, Populus trichocarpa, Proteins, Sequence Homology, Amino Acid, Sodium Chloride - pharmacology, Sucrose, Sugar, Trees